Goldman Scott J, Jin Shengkan
U.S. Army Research Institute of Environmental Medicine, Natick, MA, USA.
Methods Mol Biol. 2014;1098:129-43. doi: 10.1007/978-1-62703-718-1_11.
The imaging of spontaneously occurring tumors in mice poses many technical and logistical problems. Recently a mouse model was generated in which a chimeric protein consisting of HIF-1α oxygen-dependent degradation domain (ODD) fused to luciferase was ubiquitously expressed in all tissues. Hypoxic stress leads to the accumulation of ODD-luciferase in the tissues of this mouse model which can be identified by noninvasive bioluminescence measurement. Crossing this transgenic mouse with tumorigenic mice yields solid tumors with hypoxic cores that may be successfully imaged and characterized using the technique described herein.
对小鼠自发产生的肿瘤进行成像存在许多技术和后勤问题。最近构建了一种小鼠模型，其中一种由与荧光素酶融合的HIF-1α氧依赖性降解结构域（ODD）组成的嵌合蛋白在所有组织中普遍表达。缺氧应激导致该小鼠模型组织中ODD-荧光素酶的积累，可通过非侵入性生物发光测量来识别。将这种转基因小鼠与致瘤小鼠杂交可产生具有缺氧核心的实体瘤，使用本文所述技术可成功对其进行成像和表征。
