矮牵牛类群的分子系统发育与 DNA 扩增指纹图谱。

Molecular phylogeny and DNA amplification fingerprinting of Petunia taxa.

机构信息

Department of Ornamental Horticulture and Landscape Design, Institute of Agriculture, The University of Tennessee, 37901-1071, Knoxville, TN, USA.

出版信息

Theor Appl Genet. 1996 Jun;92(8):1009-16. doi: 10.1007/BF00224042.

DOI:10.1007/BF00224042

PMID:24166629

Abstract

The relationship of five species of Petunia and ten cultivars of the cultivated petunia, Petunia x hybrida, were investigated using DNA-amplification fingerprinting (DAF). Reproducible banding profiles were obtained from P. parodii and P. axillaris DNA from different seed sources. In contrast, other petunias such as P. inflata, P. violacea and P. integrifolia produced variable fingerprints when different plants were examined. However, representative profiles of the variable Petunia taxa were obtained by bulking the leaf tissue from ten different individual plants. Each of ten octamer primers revealed polymorphic loci between taxa. Among a total of 201 bands produced, 146 (73%) loci were polymorphic and distinguished all species and cultivars. Phenetic and cluster analysis using DAF markers separated P. axillaris from P. parodii and distinguished between the violet-flowered species, P. inflata, P. violacea, and P. integrifolia. P. parodii grouped together with the monophyletic set of the ten cultivars of P. x hybrida examined, indicating that it had made a major contribution to the development of these cultivars. Cultivars were distributed within the dendograms by flower color. The results demonstrated the utility of DAF in establishing relationships among closely related species and cultivars of Petunia.

摘要

利用 DNA 扩增指纹图谱（DAF）研究了 5 种矮牵牛属植物和 10 个栽培矮牵牛品种（Petunia x hybrida）之间的关系。从不同种子来源的 P. parodii 和 P. axillaris DNA 中获得了可重复的带型谱。相比之下，其他矮牵牛如 P. inflata、P. violacea 和 P. integrifolia 在不同植物被检测时产生了可变的指纹图谱。然而，通过将来自 10 个不同个体植物的叶片组织混合，获得了可变矮牵牛分类群的代表性图谱。每 10 个八聚体引物都揭示了分类群之间的多态性位点。在总共产生的 201 个条带中，146 个（73%）位点是多态性的，可区分所有物种和品种。使用 DAF 标记进行的表型和聚类分析将 P. axillaris 与 P. parodii 分开，并区分了紫罗兰色花的物种 P. inflata、P. violacea 和 P. integrifolia。P. parodii 与所研究的 10 个 P. x hybrida 品种的单系集聚在一起，表明它对这些品种的发展做出了重大贡献。品种根据花色分布在聚类图中。结果表明，DAF 在建立矮牵牛属植物和品种之间的关系方面具有实用性。