Estevez E G, Levine J A
J Clin Microbiol. 1985 Oct;22(4):666-7. doi: 10.1128/jcm.22.4.666-667.1985.
To review the appropriateness of standard reference procedures for diagnostic parasitology, we examined 2,206 stool specimens in our laboratory by direct wet mounting with saline and iodine, by saline and iodine wet mounting from Formalin-ethyl acetate concentrates, and by permanent staining with Wheatley's modified trichrome method (W.B. Wheatley, Am. J. Clin. Pathol. 21: 990-991, 1951). Parasites were detected in 98 stool specimens (4.4%). In all but three specimens, direct wet mounting with saline and iodine contributed little significant information to the result yet consumed substantial technical time. We recommend that with preserved feces a direct examination not be performed but that examination of both a concentrate and a permanent stain be routine.
为评估诊断寄生虫学标准参考程序的适用性，我们在实验室中采用生理盐水和碘液直接湿片法、福尔马林-乙酸乙酯浓缩物的生理盐水和碘液湿片法以及惠特利改良三色染色法（W.B.惠特利，《美国临床病理学杂志》21: 990 - 991, 1951）对2206份粪便标本进行了检测。在98份粪便标本（4.4%）中检测到了寄生虫。除3份标本外，生理盐水和碘液直接湿片法对结果贡献的重要信息很少，却耗费了大量的技术时间。我们建议，对于保存的粪便标本，不进行直接检查，而常规检查浓缩物和永久染色标本。
