National Maize and Sorghum Research Center, CNPMS/EMBRAPA, Caixa Postal 151, 35701-970, Sete Lagoas, MG, Brazil.
Theor Appl Genet. 1995 Jul;91(2):274-81. doi: 10.1007/BF00220889.
Modifier genes have been described that convert the soft endosperm of opaque2 mutants to a hard, vitreous phenotype. The mode of action and the components of the genetic system involved in this seed modification are poorly understood. We used genetic and biochemical analyses to investigate the number of opaque2 modifier genes, their mode of action and their relationship to the biochemical alterations in the modified endosperm. Using two inbred opaque2 lines, we showed that the activity of opaque2 modifier genes is influenced by the genetic background. Analysis of segregating progenies and recombinant inbred lines derived from crosses between opaque2 and modified opaque2 genotypes indicated two independent loci affecting seed opacity and density. Consistent association between endosperm modification and enhanced accumulation of the gamma-zein storage protein suggested that either this protein is directly involved in the process of seed modification, or else that a modifier gene could be tightly linked to the genes responsible for gamma-zein synthesis.
已经描述了一些修饰基因，它们可以将不透明 2 突变体的软胚乳转化为坚硬的玻璃态表型。这种种子修饰涉及的作用模式和遗传系统成分还知之甚少。我们使用遗传和生化分析来研究不透明 2 修饰基因的数量、它们的作用模式以及它们与修饰胚乳生化变化的关系。利用两个近交系不透明 2 品系，我们表明不透明 2 修饰基因的活性受遗传背景的影响。对来自不透明 2 和修饰不透明 2 基因型之间杂交的分离后代和重组近交系的分析表明，有两个独立的位点影响种子不透明度和密度。胚乳修饰与 γ-zein 贮藏蛋白积累增强之间的一致关联表明，该蛋白要么直接参与种子修饰过程，要么修饰基因可能与负责 γ-zein 合成的基因紧密连锁。
