Kang Soo-Hwan, Chung Yang-Guk, Oh Il-Hoan, Kim Yong-Sik, Min Ki-Ouk, Chung Jun-Young
Department of Orthopedic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Cell Tissue Res. 2014 Jan;355(1):81-8. doi: 10.1007/s00441-013-1738-z. Epub 2013 Oct 30.
For developing a clinically effective bone regeneration strategy, we compare the bone regeneration potential of cultured allogeneic bone marrow-derived mesenchymal stem cells (BM-MSCs) and of autologous BM-MSCs loaded onto allogeneic cancellous bone granule scaffolds. A critical-sized segmental bone defect was made at the mid-shaft of both radiuses in 19 New Zealand White rabbits (NWRs). In the experimental group, allogeneic BM-MSCs loaded onto small-sized allogeneic cancellous bone granules (300~700 um in diameter) were implanted in one side of a bone defect. In the control group, autologous BM-MSCs loaded onto allogeneic cancellous granules were grafted in the other side. Bone regeneration was assessed by radiographic evaluation at 4, 8, 12 and 16 weeks post-implantation and by micro-computed tomography (micro-CT) and histological evaluation at 8 and 16 weeks. The experimental groups showed lower bone quantity indices (BQIs) than the control groups at 12 and 16 weeks (p < 0.05), although no significant difference was observed at 4 and 8 weeks (p > 0.05). Micro-CT analysis revealed that both groups had similar mean total bone volume and other parameters including trabecular thickness, number and separation at either 8 or 16 weeks. Only bone surface area revealed less area in the experimental group at 16 weeks. Histological evaluation of 8-week and 16-week specimens showed similar biologic processes of new bone formation and maturation. There was no inflammatory reaction indicating an adverse immune response in both allogeneic and autologous MSC groups. In conclusion, allogeneic BM-MSCs loaded onto allogeneic cancellous bone granules had comparable bone regeneration potential to autologous BM-MSCs in a rabbit radial defect model.
为制定一种临床有效的骨再生策略，我们比较了培养的同种异体骨髓间充质干细胞（BM-MSCs）和负载于同种异体松质骨颗粒支架上的自体BM-MSCs的骨再生潜力。在19只新西兰白兔（NWRs）的双侧桡骨中轴制造临界尺寸的节段性骨缺损。在实验组，将负载于小尺寸同种异体松质骨颗粒（直径300~700μm）上的同种异体BM-MSCs植入骨缺损的一侧。在对照组，将负载于同种异体松质颗粒上的自体BM-MSCs移植到另一侧。在植入后4、8、12和16周通过影像学评估骨再生情况，并在8和16周通过显微计算机断层扫描（micro-CT）和组织学评估骨再生情况。实验组在12和16周时的骨量指数（BQIs）低于对照组（p<0.05），尽管在4和8周时未观察到显著差异（p>0.05）。Micro-CT分析显示，两组在8周或16周时的平均总骨体积以及包括小梁厚度、数量和间距在内的其他参数相似。仅在16周时，实验组的骨表面积显示较小。对8周和16周标本的组织学评估显示新骨形成和成熟的生物学过程相似。在同种异体和自体间充质干细胞组中均未出现表明不良免疫反应的炎症反应。总之，在兔桡骨缺损模型中，负载于同种异体松质骨颗粒上的同种异体BM-MSCs与自体BM-MSCs具有相当的骨再生潜力。
