Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada; Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, Manitoba, Canada.
Mol Nutr Food Res. 2014 Apr;58(4):768-81. doi: 10.1002/mnfr.201300332. Epub 2013 Oct 30.
Dietary fish oil (FO) and soy protein (SP) are two interventions that slow disease progression in the Han:SPRD-Cy rat model of polycystic kidney disease (PKD). Inhibition of cyclooxygenase (COX)-derived eicosanoids also reduces disease progression, but the role of lipoxygenase (LOX) products in this disease is not known.
Since dietary FO and SP have been shown to alter eicosanoid formation via differing mechanisms, Han:SPRD-Cy rats were given diets containing either casein protein (CP) or SP, and soy oil (SO) or FO. Analysis of eicosanoids revealed that renal COX products were higher and LOX products were lower in diseased kidneys. SP feeding resulted in lower COX products, activity and COX1 protein and higher LOX products in the diseased kidneys in parallel with reduced renal cyst growth and fibrosis. By comparison, FO reduced both COX and LOX products produced from n-6 fatty acids and increased 3-series prostanoids in both normal and diseased cortex and medulla, but these differences did not parallel effects on disease.
Renal COX-derived eicosanoids are elevated and LOX products are reduced in this model of kidney disease. The effects of dietary SP, but not FO, on renal eicosanoids parallel the effects on disease.
饮食中的鱼油（FO）和大豆蛋白（SP）是两种可以减缓多囊肾病（PKD）Han：SPRD-Cy 大鼠模型中疾病进展的干预措施。环氧化酶（COX）衍生的类二十烷酸的抑制作用也可减缓疾病进展，但在这种疾病中，脂氧合酶（LOX）产物的作用尚不清楚。
由于饮食中的 FO 和 SP 通过不同的机制改变了类二十烷酸的形成，因此给 Han：SPRD-Cy 大鼠喂食含有酪蛋白（CP）或 SP 以及大豆油（SO）或 FO 的饮食。对类二十烷酸的分析表明，病变肾脏中的 COX 产物更高，LOX 产物更低。SP 喂养导致病变肾脏中的 COX 产物降低，活性和 COX1 蛋白降低，同时肾脏囊肿生长和纤维化减少。相比之下，FO 降低了 n-6 脂肪酸产生的 COX 和 LOX 产物，并增加了正常和病变皮质和髓质中的 3 系列前列腺素，但这些差异与疾病的影响不平行。
在这种肾脏疾病模型中，肾脏 COX 衍生的类二十烷酸升高，LOX 产物减少。饮食中 SP 的作用（而非 FO）对肾脏类二十烷酸的影响与对疾病的影响平行。
