Neumaier M, Fenger U, Wagener C
Mol Immunol. 1985 Nov;22(11):1273-7. doi: 10.1016/0161-5890(85)90046-x.
Antigens related to the carcinoembryonic antigen (CEA) were isolated from normal human plasma by perchloric acid extraction, gel permeation chromatography and immunoaffinity chromatography using a monoclonal antibody with broad specificity and high affinity. The antigens were separated by SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose. The binding of five monoclonal anti-CEA antibodies with different epitope specificities to the immobilized antigens was analyzed. Two antigens with mol. wts of greater than 200,000 and 177,000 bound all five antibodies, and two antigens with mol. wts of 114,000 and 85,000 bound three of the five antibodies. The findings reported indicate that even monoclonal antibodies with high specificity for colonic cancer CEA detect CEA-related antigens in normal human plasma.
通过高氯酸提取、凝胶渗透色谱法和免疫亲和色谱法，使用具有广泛特异性和高亲和力的单克隆抗体，从正常人血浆中分离出与癌胚抗原（CEA）相关的抗原。通过SDS-聚丙烯酰胺凝胶电泳分离抗原并转移至硝酸纤维素膜上。分析了五种具有不同表位特异性的单克隆抗CEA抗体与固定化抗原的结合情况。分子量大于200,000和177,000的两种抗原与所有五种抗体结合，分子量为114,000和85,000的两种抗原与五种抗体中的三种结合。所报道的研究结果表明，即使是对结肠癌CEA具有高特异性的单克隆抗体也能检测出正常人血浆中的CEA相关抗原。
