Song G X, Lin C T, Wu J Y, Lam K W, Li C Y, Yam L T
Prostate. 1985;7(1):63-71. doi: 10.1002/pros.2990070108.
Immunoelectron microscopic studies were done on prostatic tissues obtained from patients with benign hyperplasia. Rabbit IgG-peroxidase conjugate against purified human prostatic acid phosphatase band 2 (HPAP-2) was used for studies. Under the light microscope, the columnar secretory epithelia of prostatic glands showed different intensity and distribution of immunostaining whereas the basal cells were unstained. Under the electron microscope, the secretory epithelial cells often showed electron-dense reaction product in the Golgi apparatus and secretory vesicles and vacuoles, and only sparingly in the cisternae of nuclear envelope and rough ER. Sometimes, fusion of secretory vacuolar membrane and plasma membrane and discharge of the vacuolar contents into the extracellular space were noted. The surfaces of microvilli at the apical portion of the columnar epithelia and the lumen of the glandular acini always showed reaction product. These findings suggest that HPAP-2 may be synthesized in the rough ER and transported to the Golgi apparatus where it is concentrated and transferred to the secretory vesicles and vacuoles. HPAP-2 is finally discharged into the extracellular spaces through exocytosis, a secretory mechanism similar to that of other secretory proteins.
对从良性前列腺增生患者获取的前列腺组织进行了免疫电子显微镜研究。使用针对纯化的人前列腺酸性磷酸酶条带2（HPAP - 2）的兔IgG - 过氧化物酶偶联物进行研究。在光学显微镜下，前列腺腺泡的柱状分泌上皮显示出不同强度和分布的免疫染色，而基底细胞未染色。在电子显微镜下，分泌上皮细胞常在高尔基体、分泌小泡和液泡中显示电子致密反应产物，而在核膜池和粗面内质网中则很少见。有时，可见分泌泡膜与质膜融合，泡内容物排入细胞外空间。柱状上皮顶端部分的微绒毛表面和腺泡腔始终显示有反应产物。这些发现表明，HPAP - 2可能在粗面内质网中合成，然后转运至高尔基体，在那里浓缩并转移至分泌小泡和液泡。HPAP - 2最终通过胞吐作用排入细胞外空间，这是一种与其他分泌蛋白类似的分泌机制。
