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木糖和甘氨酸模型体系中蓝色美拉德反应产物的优化形成及其相关抗氧化活性。

Optimised formation of blue Maillard reaction products of xylose and glycine model systems and associated antioxidant activity.

作者信息

Yin Zi, Sun Qian, Zhang Xi, Jing Hao

机构信息

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, P.R. China.

出版信息

J Sci Food Agric. 2014 May;94(7):1332-9. doi: 10.1002/jsfa.6415. Epub 2013 Oct 31.

Abstract

BACKGROUND

A blue colour can be formed in the xylose (Xyl) and glycine (Gly) Maillard reaction (MR) model system. However, there are fewer studies on the reaction conditions for the blue Maillard reaction products (MRPs). The objective of this study is to investigate characteristic colour formation and antioxidant activities in four different MR model systems and to determine the optimum reaction conditions for the blue colour formation in a Xyl-Gly MR model system, using the random centroid optimisation program.

RESULTS

The blue colour with an absorbance peak at 630 nm appeared before browning in the Xyl-Gly MR model system, while no blue colour formation but only browning was observed in the xylose-alanine, xylose-aspartic acid and glucose-glycine MR model systems. The Xyl-Gly MR model system also showed higher antioxidant activity than the other three model systems. The optimum conditions for blue colour formation were as follows: xylose and glycine ratio 1:0.16 (M:M), 0.20 mol L⁻¹ NaHCO₃, 406.1 mL L⁻¹ ethanol, initial pH 8.63, 33.7°C for 22.06 h, which gave a much brighter blue colour and a higher peak at 630 nm.

CONCLUSION

A characteristic blue colour could be formed in the Xyl-Gly MR model system and the optimum conditions for the blue colour formation were proposed and confirmed.

摘要

背景

在木糖(Xyl)和甘氨酸(Gly)美拉德反应(MR)模型体系中可形成蓝色。然而,关于蓝色美拉德反应产物(MRPs)反应条件的研究较少。本研究的目的是调查四种不同MR模型体系中的特征颜色形成和抗氧化活性,并使用随机质心优化程序确定木糖 - 甘氨酸MR模型体系中蓝色形成的最佳反应条件。

结果

在木糖 - 甘氨酸MR模型体系中,在褐变之前出现了在630 nm处有吸收峰的蓝色,而在木糖 - 丙氨酸、木糖 - 天冬氨酸和葡萄糖 - 甘氨酸MR模型体系中未观察到蓝色形成,仅观察到褐变。木糖 - 甘氨酸MR模型体系也显示出比其他三个模型体系更高的抗氧化活性。蓝色形成的最佳条件如下:木糖与甘氨酸比例1:0.16(M:M)、0.20 mol L⁻¹ NaHCO₃、406.1 mL L⁻¹乙醇、初始pH 8.63、33.7°C反应22.06 h,这产生了更亮的蓝色以及在630 nm处更高的峰值。

结论

在木糖 - 甘氨酸MR模型体系中可形成特征性蓝色,并提出并确认了蓝色形成的最佳条件。

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