Department of Biological Sciences, State University of New York at Albany, 12203, Albany, New York.
J Membr Biol. 1970 Dec;2(1):119-26. doi: 10.1007/BF01869855.
The effect of a water-soluble carbodiimide has been used to study the nature of the presumed anionic part of the acetylcholine (ACh) receptor at the frog neuromuscular junction. The ACh sensitivity has been measured by the moving fluid electrode method and by recording end plate potentials with microelectrodes. The carbodiimide blocked ACh sensitivity without marked effect on the membrane resistance or potential difference. The conditions of reversibility of the block and the results obtained with phospholipids suggest that a carboxyl group is important in the combination of ACh with the receptor.
水合碳化二亚胺的作用已被用于研究假定的青蛙神经肌肉接头处乙酰胆碱 (ACh) 受体的阴离子部分的性质。通过移动流体电极法和用微电极记录终板电位来测量 ACh 敏感性。该碳二亚胺阻断了 ACh 敏感性，而对膜电阻或电位差没有明显影响。阻断的可逆条件和与磷脂的结果表明，羧基在 ACh 与受体的结合中很重要。
