Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea ; Department of Oral Microbiology and Immunology, College of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:509482. doi: 10.1155/2013/509482. Epub 2013 Sep 23.
Chrysanthemum zawadskii Herbich var. latilobum Kitamura, known as "Gujulcho" in Korea, has been used in traditional medicine to treat various inflammatory diseases, including rheumatoid arthritis. However, these effects have not been tested on osteoclasts, the bone resorbing cells that regulate bone metabolism. Here, we investigated the effects of C. zawadskii Herbich var. latilobum Kitamura ethanol extract (CZE) on osteoclast differentiation induced by treatment with the receptor activator of NF- κ B ligand (RANKL). CZE inhibited osteoclast differentiation and formation in a dose-dependent manner. The inhibitory effect of CZE on osteoclastogenesis was due to the suppression of ERK activation and the ablation of RANKL-stimulated Ca(2+)-oscillation via the inactivation of PLC γ 2, followed by the inhibition of CREB activation. These inhibitory effects of CZE resulted in a significant repression of c-Fos expression and a subsequent reduction of NFATc1, a key transcription factor for osteoclast differentiation, fusion, and activation in vitro and in vivo. These results indicate that CZE negatively regulates osteoclast differentiation and may be a therapeutic candidate for the treatment of various bone diseases, such as postmenopausal osteoporosis, rheumatoid arthritis, and periodontitis.
菊苣 Zawadskii Herbich 变种。latilobum 菊苣，在韩国被称为“Gujulcho”，已被用于传统医学治疗各种炎症性疾病，包括类风湿关节炎。然而，这些作用尚未在破骨细胞（调节骨代谢的骨吸收细胞）上进行测试。在这里，我们研究了菊苣 Zawadskii Herbich 变种。Kitamura 乙醇提取物（CZE）对核因子-κB 配体（RANKL）诱导的破骨细胞分化的影响。CZE 以剂量依赖性方式抑制破骨细胞分化和形成。CZE 对破骨细胞生成的抑制作用是由于 ERK 激活的抑制和 PLC γ 2 的失活导致 RANKL 刺激的 Ca(2+)震荡的消融，随后抑制 CREB 激活。CZE 的这些抑制作用导致 c-Fos 表达的显著抑制，随后 NFATc1 的减少，NFATc1 是体外和体内破骨细胞分化、融合和激活的关键转录因子。这些结果表明，CZE 负调节破骨细胞分化，可能是治疗各种骨疾病（如绝经后骨质疏松症、类风湿关节炎和牙周炎）的治疗候选药物。
