[Functional morphology of nucleated erythrocytes during hypoxia (in vitro experiments)].

Changes in morphologic and functional properties of nucleic erythrocytes were observed during experimental hypoxia in vitro. It has been shown that erythrocytes volume and nuclei volume decrease in oxygen concentration of 1.76-4.03 mgO2 x l(-1) on 1.5-5.0% and 9-15% respectively. Both indexes increased significantly in case of deep hypoxia (0.57-1.76 mgO2 x l(-1)) when nucleo-cytoplasmic ratio doesn't change. By this time erythrocytes shape modifies into extended ellipse because of elongation of large axis of cell (C1) and its width (h). Along with increase in nuclei volume the enhancement of SYBR Green I fluorescence is observed; the intensity correlates with volume magnitude of this cell structure (R2 = 0.81).