Andreyeva Aleksandra Y, Soldatov Aleksander A, Mukhanov Vladimir S
Department of Animal Physiology and Biochemistry, Institute of Marine Biological Research, Russian Academy of Sciences, Leninsky Ave, 14, Moscow, Russia, 119991.
Department of Plankton, Institute of Marine Biological Research, Russian Academy of Sciences, Moscow, Russia.
In Vitro Cell Dev Biol Anim. 2017 Apr;53(4):312-319. doi: 10.1007/s11626-016-0111-4. Epub 2016 Nov 10.
The investigation of the mechanisms of red blood cell steadiness to the oxygen lack in tolerant teleosts is of current scientific interest. Black scorpionfish, Scorpaena porcus L., is a widespread benthal species in the Black Sea and is highly resistant to hypoxic influence. The morphological state of black scorpionfish red blood cells under acute hypoxia was assessed using DNA-binding dye SYBR Green I and fluorescent microscopy. Changes in membrane potential of mitochondria and functional activity of cells were determined by rhodamine 123 (R123) and fluorescein diacetate (FDA) fluorescence. Oxygen deficiency leads to bidirectional changes in volume of erythrocytes and their nuclei. Between 0.57 and 1.76 mg О l, both parameters increased on 3-12 and 7-21%, respectively. At 1.76-4.03, cells shrank on 1.5-6.0% and nucleus size decreased on 1.5-3%. Acute hypoxia induced a significant increase of R123 (12-60%) and FDA (30-184%) fluorescence. These reactions are caused by a probable decrease in erythrocyte membrane permeability.
对耐低氧硬骨鱼红细胞对缺氧稳定性机制的研究具有当前的科学意义。黑鲉，Scorpaena porcus L.，是黑海一种广泛分布的底栖物种，对缺氧影响具有高度抗性。使用DNA结合染料SYBR Green I和荧光显微镜评估了急性缺氧条件下黑鲉红细胞的形态状态。通过罗丹明123（R123）和荧光素二乙酸酯（FDA）荧光测定线粒体膜电位的变化和细胞的功能活性。缺氧导致红细胞及其细胞核体积的双向变化。在0.57至1.76 mg О l之间，这两个参数分别增加了3 - 12%和7 - 21%。在1.76 - 4.03时，细胞收缩了1.5 - 6.0%，细胞核大小减小了1.5 - 3%。急性缺氧导致R123（12 - 60%）和FDA（30 - 184%）荧光显著增加。这些反应可能是由于红细胞膜通透性降低引起的。
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