Ohashi Y, Tanaka Y, Yoshihara K, Kamiya T
Biochem Int. 1985 Oct;11(4):501-7.
The activity of purified bull seminal RNase was markedly stimulated by various basic proteins. At the half concentration of substrate RNA, basic proteins such as histones, high-mobility group chromosomal proteins and cytochrome c stimulated the enzyme activity 4-6 fold. Other non-basic proteins such as bovine serum albumin and human gamma-globulin were far less effective. In addition to enzyme-stimulating activity, basic proteins showed a marked enzyme-stabilizing activity, indicating the presence of a strong interaction between the enzyme and basic proteins.
纯化的公牛精液核糖核酸酶的活性受到多种碱性蛋白质的显著刺激。在底物RNA浓度减半时，组蛋白、高迁移率族染色体蛋白和细胞色素c等碱性蛋白质可将该酶的活性提高4至6倍。其他非碱性蛋白质，如牛血清白蛋白和人γ球蛋白的效果则要差得多。除了酶刺激活性外，碱性蛋白质还表现出显著的酶稳定活性，这表明酶与碱性蛋白质之间存在强烈的相互作用。
