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用于评估单层细胞偶联的模型方法。

Model approaches for evaluation of cell coupling in monolayers.

机构信息

Medical Physics Department, Physical Laboratory, State University of Utrecht, Utrecht, The Netherlands.

出版信息

J Membr Biol. 1972 Dec;7(1):88-110. doi: 10.1007/BF01867910.

Abstract

The electrical coupling of epithelial cells of the intestine of chick embryo cultured in monolayers was studied. This coupling can be evaluated by regarding the monolayer as a honeycomb structure of cells and the cells as equipotential spaces, as long as the cells are small (diameter <25μm). With help of this discrete model it was found that for the non-junctional membrane the membrane resistance isϱ m =250-2,000 Ω cm(2), and for the junctional membraneϱ m =5-50 Ω cm(2). In addition to this discrete model, a continuous model was also considered and good agreement between the two descriptions was found. With the aid of the continuous model, a value for the non-junctional membrane capacitance (C m ) was obtained: 5-50 μF/cm(2). The electrical values are not corrected for membrane folding, microvilli and the like. Tentative corrections based on electron microscopy suggest: 1,000 <ϱ m < 10,000 Ω cm(2), 10 <ϱ m < 100 Ω cm(2), 1<C m < 10 μF/cm(2.)

摘要

本文研究了单层培养的鸡胚肠上皮细胞的电偶联。只要细胞较小(直径 <25μm),就可以将单层视为细胞的蜂窝结构,并将细胞视为等电位空间,从而评估这种偶联。借助这个离散模型,发现非连接膜的膜电阻为ϱ m =250-2000 Ω cm(2),而连接膜的膜电阻为ϱ m =5-50 Ω cm(2)。除了这个离散模型,还考虑了一个连续模型,并发现这两种描述之间有很好的一致性。借助连续模型,获得了非连接膜电容(C m )的值:5-50 μF/cm(2)。这些电值未经膜折叠、微绒毛等因素校正。基于电子显微镜的初步校正表明:1000 <ϱ m < 10000 Ω cm(2),10 <ϱ m < 100 Ω cm(2),1<C m < 10 μF/cm(2)。

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