分离和鉴定卵菌真菌致病疫霉的线粒体 DNA。

Isolation and characterization of mitochondrial DNA of the oomycetous fungus Phytophthora infestans.

机构信息

Abt. für Molekulare Genetik der Gesellschaft für Strahlen- und Umweltforschung mbH München in Göttingen, Grisebachstr. 8, D-3400, Göttingen, Germany.

出版信息

Curr Genet. 1984 May;8(4):323-6. doi: 10.1007/BF00419731.

DOI:10.1007/BF00419731

PMID:24177802

Abstract

Mitochondrial DNA (mtDNA) of Phytophthora infestans has been isolated and preliminarily characterized. It has a low GC content of about 22.4% which is distinctly different from that of nuclear DNA (51 %). This property has been used to separate both DNA species in the presence of 4',6-diamidine-2-phenylindole (DAPI) in CsCl density gradients. The use of cetyl triammonium bromide (CTAB) for extraction of DNA significantly reduced its degradation. The base distribution of the mtDNA shows a limited intramolecular heterogeneity. The molecule contains 36.2 ± 0.3 kb as revealed by endonuclease digestion and seems to be circular as shown by restriction mapping. No differences were found in restriction patterns between mtDNAs from various pathotypes.

摘要

已分离并初步鉴定了致病疫霉的线粒体 DNA（mtDNA）。它的 GC 含量低，约为 22.4%，与核 DNA（51%）明显不同。该特性已被用于在 CsCl 密度梯度中存在 4'，6-二脒基-2-苯基吲哚（DAPI）的情况下分离两种 DNA 物质。十六烷基三甲基溴化铵（CTAB）用于 DNA 的提取，可显著减少其降解。mtDNA 的碱基分布显示出有限的分子内异质性。该分子包含 36.2±0.3kb，如内切酶消化所揭示的，并如限制图谱所示，似乎是圆形的。在来自不同致病型的 mtDNAs 之间未发现限制图谱的差异。