高分子量植物DNA的快速分离
Rapid isolation of high molecular weight plant DNA.
作者信息
Murray M G, Thompson W F
出版信息
Nucleic Acids Res. 1980 Oct 10;8(19):4321-5. doi: 10.1093/nar/8.19.4321.
Abstract
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations.
摘要
本文介绍了一种快速分离高分子量植物DNA（长度为50,000个碱基对或更长）的方法，该DNA不含干扰限制性内切核酸酶完全消化的污染物。此方法可产生总细胞DNA（即核DNA、叶绿体DNA和线粒体DNA）。该技术对于从许多不同物种中快速分离少量DNA非常理想，也适用于大规模分离。
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