Research Department; Bass PLC, 137 High Street, DE14 1JZ, Burton on Trent, Great Britain.
Curr Genet. 1984 Aug;8(6):471-5. doi: 10.1007/BF00433914.
A cloned endo-1,3-1,4-β-glucanase gene from the Gram-positive bacterium B. subtilis has been located by deletion analysis on a 1.4 kb PvuI-ClaI DNA fragment. This gene has been sub-cloned in the yeast LEU2 vector pJDB207 to produce a hybrid plasmid designated pEHB9. pEHB9 has been transformed to S. cerevisiae and shown to direct the synthesis of an endo-1,3-1,4-β-glucanase in yeast. The β-glucanase activity was low and could only be detected in crude cell extracts of yeast harbouring pEHB9.
一个来源于革兰氏阳性细菌枯草芽孢杆菌的克隆内切-1,3-1,4-β-葡聚糖酶基因，已通过缺失分析定位在一个 1.4kb 的 PvuI-ClaI DNA 片段上。这个基因已被亚克隆到酵母亮氨酸缺陷型载体 pJDB207 中，产生了一个被命名为 pEHB9 的杂合质粒。pEHB9 已转化到酿酒酵母中，并证明可以在含有 pEHB9 的酵母中指导内切-1,3-1,4-β-葡聚糖酶的合成。β-葡聚糖酶活性很低，只能在含有 pEHB9 的酵母粗细胞提取物中检测到。
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