Universität Tübingen, Botanisches Institut, Physiologische Ökologie der Pflanzen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany.
Plant Cell Rep. 1996 Sep;15(12):914-9. doi: 10.1007/BF00231587.
As part of the D-2 Spacelab mission, tobacco (Nicotiana tabacum L.) protoplasts were cultured for 10 days in microgravity and successfully regenerated into microcalli, which, after further cultivation on the ground, gave rise to intact plants. Protein analysis was performed on samples taken during the initial microgravity period and compared to ground controls. Total protein content and protein patterns were monitored, as well as the cytoskeletal proteins tubulin and actin, a key enzyme of secondary metabolism, phenylalanine ammonia lyase, and the pathogenesis-related protein osmotin. None of the investigated proteins showed a gravity-dependent effect. Since relative changes due to culture age were detectable in the immunoblots as well as in the total protein pattern, an adaptation of the cells to microgravity without major modifications of their protein complement may be assumed.
作为 D-2 航天实验室任务的一部分，烟草（Nicotiana tabacum L.）原生质体在微重力下培养了 10 天，并成功再生为微愈伤组织，在地面进一步培养后，产生了完整的植物。在初始微重力期间采集样品并进行蛋白质分析，并与地面对照进行比较。监测总蛋白含量和蛋白图谱，以及细胞骨架蛋白微管蛋白和肌动蛋白、次生代谢关键酶苯丙氨酸解氨酶和病程相关蛋白 osmotin。没有一种被调查的蛋白质表现出重力依赖性效应。由于在免疫印迹和总蛋白图谱中都可以检测到培养年龄引起的相对变化，因此可以假设细胞在没有对其蛋白质成分进行重大修改的情况下适应了微重力。
