Nishimura R, Utsunomiya T, Ide K, Tanabe K, Hamamoto T, Mochizuki M
Endocrinol Jpn. 1985 Aug;32(4):463-72. doi: 10.1507/endocrj1954.32.463.
The two kinds of glycoprotein hormone alpha subunit ectopically produced by an undifferentiated carcinoma of the left femoral region (TM-alpha) and an adenocarcinoma of the right external genitalia (FS-alpha) were examined for amino acid composition, isoelectric focusing, molecular weight, the ability to combine with standard hCG beta and affinity with lectins (Con A, Ricin and PNA). Both TM-alpha and FS-alpha exhibited immunoantigenicity similar to standard hCG alpha. Furthermore, there were no significant differences in the amino acid compositions of TM-alpha, FS-alpha or standard hCG alpha. In isoelectric focusing, while standard hCG alpha exhibited a neutral charge, both TM-alpha and FS-alpha exhibited strong negative charges. FS-alpha was as sensitive to sialidase as standard hCG alpha, whereas most of the TM-alpha exhibited resistance to sialidase. TM-alpha contains sialidase-insensitive peripheral material with a negative charge. The affinity with Ricin-Sepharose indicated that most of the FS-alpha and some of the TM-alpha may contain terminal sialic acid and the penultimate structure, Gal beta 1----4G1cNAc; the affinity with PNA-Sepharose indicated that both may also contain terminal sialic acid and the penultimate structure, Gal beta 1----3GalNAc. These observations suggest that dissimilar glycosylation processes are present in the carcinoma ectopic biosynthesis of glycoprotein hormone alpha subunit.
对由左股骨区未分化癌（TM-α）和右外生殖器腺癌（FS-α）异位产生的两种糖蛋白激素α亚基进行了氨基酸组成、等电聚焦、分子量、与标准hCG β结合能力以及与凝集素（刀豆球蛋白A、蓖麻毒素和花生凝集素）亲和力的检测。TM-α和FS-α均表现出与标准hCG α相似的免疫抗原性。此外，TM-α、FS-α或标准hCG α的氨基酸组成无显著差异。在等电聚焦中，标准hCG α呈中性电荷，而TM-α和FS-α均呈强负电荷。FS-α对唾液酸酶的敏感性与标准hCG α相同，而大多数TM-α对唾液酸酶具有抗性。TM-α含有带负电荷的对唾液酸酶不敏感的外周物质。与蓖麻毒素-琼脂糖的亲和力表明，大多数FS-α和一些TM-α可能含有末端唾液酸和倒数第二个结构Galβ1----4GlcNAc；与花生凝集素-琼脂糖的亲和力表明，两者也可能含有末端唾液酸和倒数第二个结构Galβ1----3GalNAc。这些观察结果表明，糖蛋白激素α亚基的癌异位生物合成中存在不同的糖基化过程。
