Chassaing G, Convert O, Lavielle S
Eur J Biochem. 1986 Jan 2;154(1):77-85. doi: 10.1111/j.1432-1033.1986.tb09361.x.
The three-dimensional structure of substance P has been studied by 1H-NMR, (500 MHz), and by circular dichroism (CD) in different solvents. The analysis of the different NMR parameters suggest that substance P adopts a rather extended structure in dimethylsulfoxide and pyridine. In water, besides the aggregation phenomenon, the monomeric substance P presents a complex conformational equilibrium. The addition of sodium dodecylsulfate to the aqueous solution induces, as shown by CD spectroscopy, a preferential alpha-helical conformation. And in methanol three structural conclusions may be drawn: the flexibility of the N-terminal Arg-Pro-Lys, the alpha-helical structure of Pro4-Gln5-Gln6-Phe7-Phe8 and the interaction of the C-terminal carboxamide with the primary amides from both glutamines.
通过1H-NMR（500兆赫）以及在不同溶剂中的圆二色性（CD）对P物质的三维结构进行了研究。对不同NMR参数的分析表明，P物质在二甲基亚砜和吡啶中呈相当伸展的结构。在水中，除聚集现象外，单体P物质呈现复杂的构象平衡。如CD光谱所示，向水溶液中添加十二烷基硫酸钠会诱导出优先的α-螺旋构象。在甲醇中可得出三个结构结论：N端精氨酸-脯氨酸-赖氨酸的灵活性、Pro4-Gln5-Gln6-Phe7-Phe8的α-螺旋结构以及C端羧酰胺与两个谷氨酰胺的伯酰胺之间的相互作用。
