Suzuki Takeo, Miyauchi Kenjyo, Sakaguchi Yuriko, Suzuki Tsutomu
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan.
Methods Mol Biol. 2014;1093:59-72. doi: 10.1007/978-1-62703-694-8_6.
Piwi-interacting RNAs (piRNA) are fully modified by 2'-O-methylation at their 3'-termini. This terminal methylation is required to prevent 3'-nucleotide addition, which serves as a tag for destabilization. In this chapter, we describe biochemical and mass spectrometric analyses of 2'-O-methylation at 3'-termini of piRNAs.
Piwi相互作用RNA（piRNA）在其3'末端被2'-O-甲基化完全修饰。这种末端甲基化是防止3'-核苷酸添加所必需的，而3'-核苷酸添加是一种不稳定标记。在本章中，我们描述了对piRNA 3'末端2'-O-甲基化的生化和质谱分析。
