推测 Schizosaccharomyces pombe 中的 K(+) 通道受 H(+)、K (+) 和 cAMP 在转录水平的调节。
Putative K(+) channel inSchizosaccharomyces pombe is regulated by H(+), K (+) and cAMP at transcriptional level.
机构信息
Institute of Botany, University of Bonn, Kirschallee 1, D-53115, Bonn, Federal Republic of Germany.
出版信息
Amino Acids. 1996 Dec;10(4):359-68. doi: 10.1007/BF00805863.
Living cells control their electrical responsiveness by regulating the quality and quantity of channels expressed in the plasma membrane. Regulation of transcription of the voltage-gated ion channels is an important part of the molecular basis of cell energization. However, the factors which control the expression of channels are not well understood. We studied the effect on the transcription of the voltage-gated K(+) channel in the yeastSchizosaccharomyces pombe of cations, pH, and therapeutic spasmolytic and hypotensive agents with different mechanisms of action, including accumulation of intracellular cAMP. A highly specific 122 bp domain of the K(+) channel between S5 and H5 with a 55% homology with Dros shab and mbk3 was amplified by nested PCR from chromosomal DNAS. pombe. Northern blot revealed a 1.8kb transcript. mRNA dot-blot and RNase-protected analysis revealed factors altering the K(+) channel transcription.
活细胞通过调节质膜中表达的通道的质量和数量来控制其电反应性。电压门控离子通道转录的调节是细胞兴奋的分子基础的重要组成部分。然而，控制通道表达的因素尚不清楚。我们研究了阳离子、pH 值以及具有不同作用机制的治疗性痉挛和降压药物（包括细胞内 cAMP 的积累）对酵母 Schizosaccharomyces pombe 中电压门控 K(+) 通道转录的影响。用嵌套 PCR 从染色体 DNA 中扩增了与 Dros shab 和 mbk3 具有 55%同源性的 K(+) 通道 S5 和 H5 之间的高度特异性 122bp 结构域。pombe。Northern blot 显示了一个 1.8kb 的转录物。mRNA 点印迹和 RNase 保护分析揭示了改变 K(+) 通道转录的因素。
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