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通过在玉米胚乳培养物中的表达构建和快速检测合成和修饰的毒素基因序列 CryIA（b&c）。

Construction and rapid testing of synthetic and modified toxin gene sequences CryIA (b&c) by expression in maize endosperm culture.

机构信息

Department of Biochemistry, University of Ottawa, 40 Marie Curie Private, K1N 6N5, Ottawa, Ontario, Canada.

出版信息

Plant Cell Rep. 1996 May;15(9):677-81. doi: 10.1007/BF00231923.

DOI:10.1007/BF00231923
PMID:24178609
Abstract

The synthesis of two modified genes, Cry IA(b) and CryIA(c), each consisting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The synthetic CryIA(c) gene was put under the control of a maize ubiquitin promoter. This construct was tested in a maize endosperm-derived suspension culture system. The use of maize endosperm culture as a quick and efficient system to test the activity of synthetic genes is described.

摘要

本文详细描述了两个修饰基因 CryIA(b) 和 CryIA(c) 的合成，每个基因都由 1845 个碱基对组成。这些基因是使用基于递归原理的改良 PCR 程序合成的。合成的 CryIA(c) 基因受玉米泛素启动子的控制。该构建体在玉米胚乳衍生的悬浮培养系统中进行了测试。本文还描述了使用玉米胚乳培养作为快速有效的系统来测试合成基因活性的方法。

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本文引用的文献

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Characterization of a maize endosperm culture expressing zein genes and its use in transient transformation assays.玉米胚乳培养体系表达 Zein 基因及其在瞬时转化检测中的应用
Plant Cell Rep. 1991 Feb;9(10):544-8. doi: 10.1007/BF00232328.
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A homologous expression system for cloned zein genes.克隆玉米醇溶蛋白基因的同源表达系统。
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Transgenic tomato line expressing modified Bacillus thuringiensis cry1Ab gene showing complete resistance to two lepidopteran pests.表达修饰的苏云金芽孢杆菌cry1Ab基因的转基因番茄品系对两种鳞翅目害虫表现出完全抗性。
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Plant Cell Rep. 2011 Sep;30(9):1603-16. doi: 10.1007/s00299-011-1071-5. Epub 2011 Apr 23.
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Transgenic rice plants expressing a modified cry1Ca1 gene are resistant to Spodoptera litura and Chilo suppressalis.转 Cry1Ca1 基因水稻对斜纹夜蛾和二化螟具有抗性。
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Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus).用于抗斑点螟（Chilo partellus）的抗虫转基因高粱的培育。
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Expression of modified Cry1Ac gene of Bacillus thuringiensis in transgenic tobacco plants.苏云金芽孢杆菌修饰的Cry1Ac基因在转基因烟草植株中的表达。
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EMBO J. 1987 Dec 20;6(13):3901-7. doi: 10.1002/j.1460-2075.1987.tb02730.x.
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Modification of the coding sequence enhances plant expression of insect control protein genes.编码序列的修饰增强了昆虫控制蛋白基因在植物中的表达。
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3324-8. doi: 10.1073/pnas.88.8.3324.
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