小鳞茎培养中非洲菊（Gerbera jamesonii）的愈伤组织诱导、单倍体植株再生和染色体加倍。

Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii).

机构信息

Sakata Seed Corp., Kakegawa Breeding Station, Kakegawa, 436-01, Shizuoka, Japan.

出版信息

Plant Cell Rep. 1996 Nov;16(1-2):1-5. doi: 10.1007/BF01275438.

Abstract

Gynogenetic plants of pot gerbera (Gerbera jamesonii) were successfully produced from cultures of unpollinated ovulesin vitro. Genotypic variations in the number of ovules that formed callus were found among the lines tested. One particularly responsive genotype was found among 17 genotypes tested where the frequency of callus-forming ovules was 17.5%. Four genotypes formed no callus at all. The frequency of shoot formation from the callus varied from 0-19.6% in nine genotypes. Ploidy was determined by flow cytometry, and 37 (80.4%) regenerants were haploid, seven (15.2%) were diploid, and two (4.3%) were mixoploid with both haploid and diploid cells. The doubling of chromosomes was achieved by treatment with 0.05% colchicine for 2-6 din vitro, and 24.2-34.1% of treated haploid plants were found to have been diploidized.

摘要

通过未授粉胚珠的离体培养，成功获得了大丁草属（大丁草）的雌核发育植株。在所测试的品系中，胚珠形成愈伤组织的数量存在基因型变异。在测试的 17 个基因型中，有一个特别敏感的基因型，其形成愈伤组织的胚珠频率为 17.5%。有 4 个基因型根本没有形成愈伤组织。在 9 个基因型中，从愈伤组织形成芽的频率从 0-19.6%不等。通过流式细胞术确定倍性，37 个（80.4%）再生体为单倍体，7 个（15.2%）为二倍体，2 个（4.3%）为混倍体，既有单倍体细胞又有二倍体细胞。通过在体外用 0.05%秋水仙素处理 2-6 天来实现染色体加倍，发现 24.2-34.1%的处理单倍体植物被二倍体化。