IgE抗体产生中的抗原竞争。III. 对Th细胞和B细胞的抑制作用。

Antigenic competition in IgE antibody production. III. Suppressive effect on Th and B cells.

作者信息

Soares M F, de Macedo M S, Perini A, Mota I

出版信息

Int Arch Allergy Appl Immunol. 1986;79(2):196-201. doi: 10.1159/000233970.

Abstract

An adoptive cell transfer system was utilized to evaluate the site of action of the suppressive mechanism involved in antigenic competition in IgE antibody production. Carrier-primed (OA) and hapten-primed (DNP-KLH) spleen cells were transferred to syngeneic irradiated recipients that were challenged with a heterologous conjugate (DNP-OA). To study the effect of antigenic competition on T and B cells, donor mice of one or the other cell type received in addition the competitor antigen (Asc) at immunization. The adoptive secondary IgE anti-DNP antibody response was suppressed in both situations. This effect could not be attributed to transfer of Asc-primed cells. Irradiation of donor mice before immunization with the two antigens abrogated the suppressive effect. These results indicate that both Th and B cells primed to the test antigen were affected by antigenic competition.

摘要

采用过继性细胞转移系统来评估参与IgE抗体产生中抗原竞争的抑制机制的作用位点。用载体致敏（OA）和半抗原致敏（DNP-KLH）的脾细胞转移至同基因照射的受体，并用异源偶联物（DNP-OA）对其进行攻击。为研究抗原竞争对T细胞和B细胞的影响，在免疫时，其中一种细胞类型的供体小鼠另外接受了竞争抗原（Asc）。在两种情况下，过继性二次IgE抗DNP抗体反应均受到抑制。这种效应不能归因于Asc致敏细胞的转移。在用两种抗原免疫前对供体小鼠进行照射可消除抑制效应。这些结果表明，被测试抗原致敏的Th细胞和B细胞均受抗原竞争的影响。