Okudaira H, Terada E, Yokoyama M, Miyamoto T
Department of Medicine and Physical Therapy, University of Tokyo.
Int Arch Allergy Appl Immunol. 1988;85(4):462-6. doi: 10.1159/000234552.
Tetraparental chimera mice were developed by aggregation of IgE high responder (DBA/2) and IgE low responder (SJL) embryos. Anti-dinitrophenyl (DNP) IgE antibody response in such mice (SJL----DBA/2) upon challenge with DNP-keyhole-limpet hemocyanin (KLH) in alum was clearly suppressed, while anti-DNP IgG antibody response was not. High-titer anti-DNP IgE and IgG antibody response developed in F1 hybrid mice of SJL and DBA/2 (SDF1) mice. The experimental results suggest that high IgE antibody production is the dominant trait, and the IgE-specific suppressor gene in SJL mice is autosomal recessive. IgE-specific suppressor T cells in SJL mice actively suppressed IgE antibody formation by DBA/2 immuno-competent cells across the histocompatibility barrier. Hapten-specific B cells and carrier-specific T cells were prepared in SJL----DBA/2 and SDF1 mice by immunization with DNP-KLH or ovalbumin (OA) in alum and transferred to irradiated SDF1 mice followed by challenge with DNP-OA. Hapten-specific B cells and carrier-specific helper T cells clearly developed in SDF1 mice. Recipient mice transferred with DNP-KLH-primed SDF1 spleen cells and OA-primed SDF1 spleen cells showed high-titer anti-DNP IgE and IgG antibody responses. OA-primed SJL----DBA/2 spleen cells cotransferred with DNP-KLH-primed SDF1 spleen cells and OA-primed SDF1 spleen cells completely abolished secondary anti-DNP IgE antibody response. The data suggest that carrier-specific helper T cells for IgE and IgG antibody responses are distinct. The regulatory role of IgE-isotype-specific suppressor cells were considered to be the interference of cooperative cellular interaction between IgE B cells and carrier-specific, IgE-specific helper T cells.
通过聚合IgE高反应性（DBA/2）和IgE低反应性（SJL）胚胎培育出了四亲嵌合小鼠。用明矾中的二硝基苯基（DNP）-钥孔戚血蓝蛋白（KLH）攻击时，此类小鼠（SJL----DBA/2）的抗二硝基苯基（DNP）IgE抗体反应明显受到抑制，而抗DNP IgG抗体反应则未受抑制。SJL和DBA/2（SDF1）小鼠的F1杂交小鼠中产生了高滴度的抗DNP IgE和IgG抗体反应。实验结果表明，高IgE抗体产生是显性性状，SJL小鼠中的IgE特异性抑制基因是常染色体隐性的。SJL小鼠中的IgE特异性抑制性T细胞通过组织相容性屏障，积极抑制DBA/2免疫活性细胞形成IgE抗体。通过用明矾中的DNP-KLH或卵清蛋白（OA）免疫，在SJL----DBA/2和SDF1小鼠中制备了半抗原特异性B细胞和载体特异性T细胞，并将其转移到经辐照的SDF1小鼠中，随后用DNP-OA攻击。SDF1小鼠中明显产生了半抗原特异性B细胞和载体特异性辅助性T细胞。用DNP-KLH致敏的SDF1脾细胞和OA致敏的SDF1脾细胞转移的受体小鼠表现出高滴度的抗DNP IgE和IgG抗体反应。与DNP-KLH致敏的SDF1脾细胞和OA致敏的SDF1脾细胞共转移的OA致敏的SJL----DBA/2脾细胞完全消除了二次抗DNP IgE抗体反应。数据表明，针对IgE和IgG抗体反应的载体特异性辅助性T细胞是不同的。IgE同种型特异性抑制细胞的调节作用被认为是干扰IgE B细胞与载体特异性、IgE特异性辅助性T细胞之间的协同细胞相互作用。
