Chai Xiao, Zhao Ming-feng, Li De-guan, Meng Juan-xia, Lu Wen-yi, Mu Juan, Meng Ai-min
Department of Hematology, Tianjin First Central Hospital, the First Central Clinical College of Tianjin Medical University, Tianjin 300192, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2013 Oct;35(5):547-52. doi: 10.3881/j.issn.1000-503X.2013.05.012.
To establish a mouse model of iron overload by intraperitoneal injection of iron dextran and investigate the impact of iron overload on bone marrow hematopoiesis.
A total of 40 C57BL/6 mice were divided into control group, low-dose iron group (12.5 mg/ml), middle-dose iron group (25 mg/ml), and high-dose iron group (50 mg/ml). The control group received normal saline (0.2 ml), and the rest were injected with intraperitoneal iron dextran every three days for six weeks. Iron overload was confirmed by observing the bone marrow, hepatic, and splenic iron deposits and the bone marrow labile iron pool. In addition, peripheral blood and bone marrow mononuclear cells were counted and the hematopoietic function was assessed.
Iron deposits in bone marrow, liver, and spleen were markedly increased in the mouse models. Bone marrow iron was deposited mostly within the matrix with no significant difference in expression of labile iron pool.Compared with control group, the ability of hematopoietic colony-forming in three interventional groups were decreased significantly (P<0.05). Bone marrow mononuclear cells counts showed no significant difference. The amounts of peripheral blood cells (white blood cells, red blood cells, platelets, and hemoglobin) in different iron groups showed no significant difference among these groups;although the platelets were decreased slightly in low-dose iron group [(780.7±39.60)×10(9)/L], middle dose iron group [(676.2±21.43)×10(9)/L], and high-dose iron group [(587.3±19.67)×10(9)/L] when compared with the control group [(926.0±28.23)×10(9)/L], there was no significant difference(P>0.05).
The iron-overloaded mouse model was successfully established by intraperitoneal administration of iron dextran. Iron overload can damage the hepatic, splenic, and bone marrow hematopoietic function, although no significant difference was observed in peripheral blood count.
通过腹腔注射右旋糖酐铁建立铁过载小鼠模型，并研究铁过载对骨髓造血的影响。
将40只C57BL/6小鼠分为对照组、低剂量铁组（12.5 mg/ml）、中剂量铁组（25 mg/ml）和高剂量铁组（50 mg/ml）。对照组给予生理盐水（0.2 ml），其余组每三天腹腔注射一次右旋糖酐铁，共六周。通过观察骨髓、肝脏和脾脏的铁沉积以及骨髓不稳定铁池来确认铁过载。此外，对外周血和骨髓单个核细胞进行计数，并评估造血功能。
小鼠模型中骨髓、肝脏和脾脏的铁沉积明显增加。骨髓铁主要沉积在基质内，不稳定铁池的表达无显著差异。与对照组相比，三个干预组的造血集落形成能力显著降低（P<0.05）。骨髓单个核细胞计数无显著差异。不同铁组外周血细胞（白细胞、红细胞、血小板和血红蛋白）数量在各组间无显著差异；虽然低剂量铁组[（780.7±39.60）×10⁹/L]、中剂量铁组[（676.2±21.43）×10⁹/L]和高剂量铁组[（587.3±19.67）×10⁹/L]的血小板数量与对照组[（926.0±28.23）×10⁹/L]相比略有下降，但差异无统计学意义（P>0.05）。
通过腹腔注射右旋糖酐铁成功建立了铁过载小鼠模型。铁过载可损害肝脏、脾脏和骨髓的造血功能，尽管外周血细胞计数未观察到显著差异。
