Chemical Engineering Department, Universitat Autònoma de Barcelona, Campus Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain.
Chemical Engineering Department, Universitat Autònoma de Barcelona, Campus Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain.
J Biosci Bioeng. 2014 Apr;117(4):471-7. doi: 10.1016/j.jbiosc.2013.09.014. Epub 2013 Oct 30.
The increasing demand for biopharmaceuticals produced in mammalian cells has lead industries to enhance bioprocess volumetric productivity through different strategies. Among those strategies, cell culture media development is of major interest. In the present work, several commercially available culture media for Human Embryonic Kidney cells (HEK293) were evaluated in terms of maximal specific growth rate and maximal viable cell concentration supported. The main objective was to provide different cell culture platforms which are suitable for a wide range of applications depending on the type and the final use of the product obtained. Performing simple media supplementations with and without animal derived components, an enhancement of cell concentration from 2 × 10(6) cell/mL to 17 × 10(6) cell/mL was achieved in batch mode operation. Additionally, the media were evaluated for adenovirus production as a specific application case of HEK293 cells. None of the supplements interfered significantly with the adenovirus infection although some differences were encountered in viral productivity. To the best of our knowledge, the high cell density achieved in the work presented has never been reported before in HEK293 batch cell cultures and thus, our results are greatly promising to further study cell culture strategies in bioreactor towards bioprocess optimization.
由于对哺乳动物细胞生产的生物制药的需求不断增加，各行业通过不同策略来提高生物工艺的体积产率。在这些策略中，细胞培养基的开发是主要关注点。在本工作中，从最大比生长速率和最大活细胞浓度两个方面评估了几种市售的人胚肾细胞（HEK293）培养基。主要目标是提供不同的细胞培养平台，这些平台适用于广泛的应用，具体取决于所获得产品的类型和最终用途。通过对含或不含动物源成分的培养基进行简单的补充，在分批操作中实现了细胞浓度从 2×10(6)个细胞/mL 到 17×10(6)个细胞/mL 的提高。此外，还评估了这些培养基用于生产腺病毒的情况，腺病毒是 HEK293 细胞的一个特定应用案例。尽管在病毒产率方面存在一些差异，但补充剂均未对腺病毒感染产生显著干扰。据我们所知，在 HEK293 批细胞培养中，以前从未报道过如此高的细胞密度，因此，我们的结果对于进一步研究生物反应器中的细胞培养策略以实现生物工艺优化具有很大的前景。
