Martin L J, Doebler J A, Anthony A
Proc Soc Exp Biol Med. 1986 Jan;181(1):41-8. doi: 10.3181/00379727-181-42222.
Neuronal nucleic acid responses were examined within the rat striatum and sensorimotor cortex (layer V) following single intraperitoneal injections of the central cholinergic-muscarinic agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric Feulgen and azure B staining of brain sections, scanning-integrating microdensitometry was used to quantify Feulgen-deoxyribonucleic acid levels, changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) and azure B-ribonucleic acid (RNA) content of neurons on an individual basis. Changes in neuronal nuclear and nucleolar volumes were also determined histometrically. Within the striatum and sensorimotor cortex, oxotremorine produced marked dose-dependent elevations in both F-DNA yield and RNA content. These metabolic increases were typically paralleled by elevations in nuclear and nucleolar volumes. The data demonstrate that the oxotremorine-induced central muscarinic activation is associated with dose-related enhancements in neuronal chromatin template activity, RNA content, and protein synthetic capacity.
在大鼠腹腔注射一次中枢胆碱能 - 毒蕈碱激动剂氧化震颤素（0.1、0.7或1.0mg/kg）后，检测纹状体和感觉运动皮层（V层）内的神经元核酸反应。在对脑切片进行化学计量的福尔根和天青B染色后，使用扫描积分显微密度计对福尔根 - 脱氧核糖核酸水平、染色质对福尔根酸水解的敏感性变化（F - DNA产量）以及单个神经元的天青B - 核糖核酸（RNA）含量进行定量分析。还通过组织测量法确定神经元细胞核和核仁体积的变化。在纹状体和感觉运动皮层内，氧化震颤素使F - DNA产量和RNA含量均出现明显的剂量依赖性升高。这些代谢增加通常与细胞核和核仁体积的增大同时出现。数据表明，氧化震颤素诱导的中枢毒蕈碱激活与神经元染色质模板活性、RNA含量和蛋白质合成能力的剂量相关增强有关。
