在生姜（Zingiber officinale Rosc.）中进行体外微根茎生产。

In vitro microrhizome production in Zingiber officinale Rosc.

机构信息

Biotechnology Centre, Himachal Pradesh Krishi Vishvavidyalaya, 176062, Palampur, India.

出版信息

Plant Cell Rep. 1995 Dec;15(3-4):274-7. doi: 10.1007/BF00193735.

DOI:10.1007/BF00193735

Abstract

Microrhizomes of Zingiber officinale were successfully produced from tissue culture derived shoots by transferring them to liquid MS medium supplemented with 1 mg/l BAP, 2 mg/l calcium pantothenate, 0.2 mg/l GA3 and 0.05 mg/l NAA for shoot proliferation. After 4 weeks of incubation, the medium was replaced with microrhizome induction medium, consisting of MS salts supplemented with 8 mg/l BAP and 75 g/l sucrose. Microrhizome formation started after 20 d of incubation in stationary cultures at 25+1 ° in the dark. Microrhizomes with 1-4 buds and weighing 73.8 to 459 mg each were harvested after 50-60 d. After storage for 2 months in moist sand at room temperature, 80% of the microrhizomes sprouted producing roots and shoots.

摘要

从组织培养衍生的芽中成功地产生了生姜的小根状茎，将其转移到添加了 1 mg/L BAP、2 mg/L 泛酸钙、0.2 mg/L GA3 和 0.05 mg/L NAA 的液体 MS 培养基中，以促进芽的增殖。经过 4 周的培养，将培养基更换为小根状茎诱导培养基，该培养基由 MS 盐补充 8 mg/L BAP 和 75 g/L 蔗糖组成。在黑暗中于 25+1°C 的静置培养中培养 20 天后，开始形成小根状茎。经过 50-60 天的培养，每个小根状茎带有 1-4 个芽，重量为 73.8 至 459 毫克。在室温下的湿润沙中储存 2 个月后，80%的小根状茎发芽，长出根和芽。