基于 RFLP 的水稻广亲和品种系统发育分析

RFLP-based phylogenetic analysis of wide compatibility varieties in Oryza sativa L.

机构信息

Department of Biotechnology, China National Rice Research Institute, 310006, Hangzhou, China.

出版信息

Theor Appl Genet. 1994 Apr;88(1):65-9. doi: 10.1007/BF00222395.

Abstract

Twenty-one wide compatibility varieties (WCVs) of rice together with three indica and three japonica testers were assayed with 160 DNA probes that were selected to represent the entire RFLP map at an average interval of 11 cM. On the basis of four enzyme digestion 125 probes detected polymorphisms among the WCVs and subspecies' testers. Among these polymorphic probes there were 68 that could distinguish the indica from the japonica testers. Two dendrograms were constructed on the basis of 398 polymorphic fragments of 125 probes and 139 polymorphic fragments of 68 subspecies' differentiating probes in combination with single enzymes, respectively. The reliability and representativeness of the testers and the levels of DNA variations among WCVs were estimated. The potential of WCVs in the utilization of intersubspecific heterosis is discussed.

摘要

21 个广亲和品种（WCVs）与三个籼稻和三个粳稻测验种一起用 160 个 DNA 探针进行了分析，这些探针被选择代表整个 RFLP 图谱，平均间隔为 11cM。基于四种酶消化，125 个探针在 WCVs 和亚种测验种之间检测到多态性。在这些多态性探针中，有 68 个可以区分籼稻和粳稻测验种。根据 125 个探针的 398 个多态性片段和 68 个亚种区分探针的 139 个多态性片段，结合单酶，分别构建了两个聚类图。估计了测验种的可靠性和代表性以及 WCVs 之间 DNA 变异的水平。讨论了 WCVs 在利用亚种间杂种优势中的潜力。