Zhang Peng, Zhong Kaizhen, Tong Hanhua, Shahid Muhammad Qasim, Li Jinquan
State Key Laboratory of Rice Biology, China National Rice Research InstituteHangzhou, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural UniversityGuangzhou, China.
State Key Laboratory of Rice Biology, China National Rice Research Institute Hangzhou, China.
Front Plant Sci. 2016 Oct 4;7:1415. doi: 10.3389/fpls.2016.01415. eCollection 2016.
Trivalent aluminum (Al) has drastic effect on the rice production in acidic soils. Elite genes for aluminum (Al) tolerance might exist in rice landraces. Therefore, the purpose of this research is to mine the elite genes within rice landraces. Association mapping for Al tolerance traits [i.e., relative root elongation (RRE)] was performed by using a core collection of 150 accessions of rice landraces (i.e., Ting's rice core collection). Our results showed that the Ting's rice core collection possessed a wide-range of phenotypic variation for Al tolerance, and the index of Al tolerance (RRE) was ranged from 0.22 to 0.89. Moreover, the groups with different origins and compositions of and rice showed different degrees of tolerance to varying levels of Al. These rice landraces were further screened with 274 simple sequence repeat markers, and association mapping was performed using a mixed linear model approach. The mapping results showed that a total of 23 significant ( < 0.05) trait-marker associations were detected for Al tolerance. Of these, three associations (13%) were identical to the quantitative trait loci reported previously, and other 20 associations were reported for the first time in this study. The proportion of phenotypic variance () explained by 23 significant associations ranged from 5.03 to 20.03% for Al tolerance. We detected several elite alleles for Al tolerance based on multiple comparisons of allelic effects, which could be used to develop Al tolerant rice cultivars through marker-assisted breeding.
三价铝（Al）对酸性土壤中的水稻产量有显著影响。耐铝（Al）的优良基因可能存在于水稻地方品种中。因此，本研究的目的是挖掘水稻地方品种中的优良基因。利用由150份水稻地方品种组成的核心种质库（即丁氏水稻核心种质库）对耐铝性状[即相对根伸长（RRE）]进行了关联分析。我们的结果表明，丁氏水稻核心种质库在耐铝性方面具有广泛的表型变异，耐铝指数（RRE）范围为0.22至0.89。此外，籼稻和粳稻不同起源和组成的群体对不同水平铝的耐受程度不同。用274个简单序列重复标记对这些水稻地方品种进行进一步筛选，并采用混合线性模型方法进行关联分析。分析结果表明，共检测到23个与耐铝性显著相关（<0.05）的性状-标记关联。其中，三个关联（13%）与先前报道的数量性状位点相同，另外20个关联是本研究首次报道。23个显著关联解释的表型变异比例（）在耐铝性方面为5.03%至20.03%。基于等位基因效应的多重比较，我们检测到了几个耐铝的优良等位基因，可用于通过标记辅助育种培育耐铝水稻品种。
