酵母脆性突变株摄取 DNA。
Uptake of DNA by fragile mutants of Saccharomyces cerevisiae.
机构信息
Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria.
出版信息
Curr Genet. 1982 Jul;5(2):153-5. doi: 10.1007/BF00365707.
When Saccharomyces cerevisiae SY15 rho° mutant cells grown in media stabilized with 10% sorbitol were suspended in 2% sorbitol solutions, 60-70% of the population did not lyse and became permeable to native high molecular weight DNA. Maximal incorporation of DNA to DNase resistant state was measured after 60 min of incubation in presence of 5 μg/ml DNA and 10 mM CaCl2. These results suggest that the fragile mutants might be tested as hosts for transformation of whole yeast cells.
当在稳定在 10%山梨醇的培养基中生长的酿酒酵母 SY15 rho°突变细胞悬浮在 2%山梨醇溶液中时，60-70%的细胞不会裂解，并对天然高分子量 DNA 具有渗透性。在存在 5μg/ml DNA 和 10 mM CaCl2 的情况下孵育 60 分钟后，测量 DNA 最大掺入到 DNase 抗性状态。这些结果表明，脆弱突变体可能被测试为整个酵母细胞转化的宿主。
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