Jing Lu, Xiao-Feng Li, Li-Xia Kong, Su-Huan Liao, Department of Gastroenterology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai 519000, Guangdong Province, China.
World J Gastroenterol. 2013 Oct 28;19(40):6883-7. doi: 10.3748/wjg.v19.i40.6883.
To investigate the mRNA expression of cyclooxygensae-2 (COX-2) in benign and malignant ascites, and to explore the difference in COX-2 mRNA expression among different diseases.
A total of 36 samples were collected from the Fifth Affiliated Hospital of Sun Yat-Sen University and divided into two experimental groups: benign ascites (n = 21) and malignant ascites (n = 15). Benign ascites included cirrhotic ascites (n = 10) and tuberculous ascites (n = 5). Malignant ascites included oophoroma (n = 7), cancer of colon (n = 5), cancer of the liver (n = 6), gastric cancer (n = 2), and bladder carcinoma (n = 1). The mRNA expression of COX-2 in ascites was examined with reverse transcriptase polymerase chain reaction (RT-PCR) technology, and the positive rate of COX-2 mRNA was compared between different diseases.
The positive rate of COX-2 mRNA in malignant ascites was 42.9% (9/21), which was significantly higher than in benign ascites, 6.7% (1/15), difference being significant between these two groups (χ(2) = 4.051, P = 0.044). The proportion of the positive rate in the malignant ascites was as follows: ovarian cancers 57.1% (4/7), colon cancer 40.0% (2/5), liver cancer 33.3% (2/6), gastric cancer 50.0% (1/2), and bladder cancer 0.00% (0/1). However, there was no significant difference in COX-2 mRNA expression among various tumors with malignant ascites (χ(2) = 1.614, P = 0.806). Among the benign ascites, COX-2 mRNA levels were different between the tuberculous ascites (0/5) and cirrhotic ascites (1/10), but there was no significant difference (P = 1.000).
COX-2 mRNA, detected by RT-PCR, is useful in the differential diagnosis of benign and malignant ascites, which also has potential value in the clinical diagnosis of tumors.
研究环氧化酶-2（COX-2）在良性和恶性腹水患者中的 mRNA 表达，并探讨不同疾病 COX-2 mRNA 表达的差异。
收集中山大学第五附属医院的 36 例标本，分为两组实验：良性腹水（n = 21）和恶性腹水（n = 15）。良性腹水包括肝硬化腹水（n = 10）和结核性腹水（n = 5）。恶性腹水包括卵巢癌（n = 7）、结肠癌（n = 5）、肝癌（n = 6）、胃癌（n = 2）和膀胱癌（n = 1）。采用逆转录聚合酶链反应（RT-PCR）技术检测腹水 COX-2 的 mRNA 表达，比较不同疾病 COX-2 mRNA 的阳性率。
恶性腹水组 COX-2 mRNA 的阳性率为 42.9%（9/21），明显高于良性腹水组的 6.7%（1/15），两组差异有统计学意义（χ² = 4.051，P = 0.044）。恶性腹水组 COX-2 mRNA 阳性率分别为卵巢癌 57.1%（4/7）、结肠癌 40.0%（2/5）、肝癌 33.3%（2/6）、胃癌 50.0%（1/2）和膀胱癌 0.00%（0/1）。然而，恶性腹水组不同肿瘤之间 COX-2 mRNA 表达无显著差异（χ² = 1.614，P = 0.806）。在良性腹水中，结核性腹水（0/5）与肝硬化腹水（1/10）之间 COX-2 mRNA 水平不同，但差异无统计学意义（P = 1.000）。
RT-PCR 检测 COX-2 mRNA 有助于鉴别良恶性腹水，对肿瘤的临床诊断有一定价值。
