苯巴比妥诱导大鼠肝脏中α2u球蛋白mRNA的表达：一个cDNA克隆的特性分析

Induction of alpha 2u globulin mRNA by phenobarbital in rat liver: characterization of a cDNA clone.

作者信息

Osorio-Almeida M L, Sinogas C, Ludovice M, Lechner M C

出版信息

Biochem Biophys Res Commun. 1986 Feb 13;134(3):1182-9. doi: 10.1016/0006-291x(86)90375-x.

Abstract

A clone has been selected from a cDNA library previously constructed from phenobarbital pre-treated rat liver polysomal poly(A)+ RNA, which was reverse transcribed. The double-stranded cDNA was inserted by GC homopolymeric tailing in the Pst I site of pAT 153, and further cloned in E. coli HB101. This clone, called 2A9, corresponds to a mRNA whose concentration is increased five fold 16 h after phenobarbital treatment. Its length is 1200 nucleotides as revealed by RNA dot and Northern blot analysis respectively. The two strands of a 450 bp fragment from the 2A9 580 bp double-stranded cDNA insert have been sequenced and proven to correspond to alpha 2u globulin mRNA. It shows one single bp difference from the sequence previously published by Unterman et al. (1981, PNAS, 78, 3478). Thus, alpha 2u globulin, a hormone regulated gene product, is inducible by phenobarbital.

摘要

从一个cDNA文库中筛选出了一个克隆，该文库是先前用苯巴比妥预处理的大鼠肝脏多聚核糖体多聚腺苷酸（poly(A)+）RNA构建的，经过逆转录处理。双链cDNA通过GC同聚物加尾插入到pAT 153的Pst I位点，并进一步克隆到大肠杆菌HB101中。这个名为2A9的克隆对应一种mRNA，其浓度在苯巴比妥处理16小时后增加了五倍。通过RNA斑点分析和Northern印迹分析分别显示其长度为1200个核苷酸。对来自2A9 580 bp双链cDNA插入片段的一个450 bp片段的两条链进行了测序，证明其对应于α2u球蛋白mRNA。它与Unterman等人（1981年，《美国国家科学院院刊》，78卷，3478页）先前发表的序列有一个单碱基差异。因此，α2u球蛋白作为一种激素调节的基因产物，可被苯巴比妥诱导。