不同实验室酵母菌株核糖体蛋白 L8 的二态性。

Dimorphism of ribosomal protein L8 among different laboratory strains of Saccharomyces cerevisiae.

机构信息

Department of Biology, Faculty of Sience, Konan University, Okamoto, 658, Kobe, Japan.

出版信息

Curr Genet. 1980 Dec;2(3):211-4. doi: 10.1007/BF00435688.

Abstract

Two-dimensional gel electrophoresis was used in a comparative study of ribosomal proteins from various strains of Saccharomyces cerevisiae. The results demonstrated a case of dimorphism of L8 protein of 60S ribosomal subunit. Of eight strains examined, two strains were one type and six were the other type. The former, which was tentatively designated as altered (A) type, was more acidic than the latter, common (C) type, as shown by mobility difference in pH gradient gel. Heterozygous (A/C) diploid cells contained both types of L8 protein and gave rise to tetrads of 2:2 segregation for A and C types, indicating that the difference of mobility was reflection of the allelic difference of the gene coding for L8 protein.

摘要

二维凝胶电泳被用于比较来自不同酿酒酵母菌株的核糖体蛋白。结果表明，60S 核糖体亚基的 L8 蛋白存在二态性。在所检查的 8 株菌中，有 2 株为一种类型，6 株为另一种类型。前者被暂时指定为改变（A）型，比后者更酸性，常见（C）型，如在 pH 梯度凝胶中的迁移率差异所示。杂合（A/C）二倍体细胞含有两种类型的 L8 蛋白，并产生 A 和 C 型的 2:2 分离四分体，表明迁移率的差异反映了编码 L8 蛋白的基因的等位基因差异。