Botanical Institute, Faculty of Science, Hiroshima University, 724, Higashi-Hiroshima, Japan.
Theor Appl Genet. 1993 Oct;87(1-2):38-43. doi: 10.1007/BF00223741.
Phylogenetic relationships among eight taxa of seven species of Phaseolus and Vigna (Phaseolus angularis, P. aureus, P. calcaratus, P. coccineus, P. vulgaris, Vigna sesquipedalis and V. sinensis; 2n = 22 each) were studied by the fluorescent chromosome banding technique. Preparations of somatic metaphase chromosomes of each taxon were sequentially stained with Giemsa, GC-specific fluorochrome chromomycin A3 (CMA) and AT-specific fluorochrome 4'-6-diamidino-2-phenylindole (DAPI). On the basis of the fluorescent banding patterns of the 22 chromosomes of each taxon, P. angularis, P. coccineus (from China and Korea) and P. vulgaris were grouped into one group ("Phaseolus group"), P. aureus and two Vigna species were grouped into another ("Vigna group") and P. calcaratus was grouped in an independent group.
利用荧光染色体分带技术研究了七个物种的八种菜豆属（Phaseolus）和豇豆属（Vigna）植物（菜豆（Phaseolus angularis）、P. aureus、P. calcaratus、P. coccineus、P. vulgaris、Vigna sesquipedalis 和 V. sinensis；每个物种的 2n = 22）的系统发育关系。每个分类群的体细胞中期染色体的制备依次用吉姆萨（Giemsa）、GC 特异性荧光染料色霉素 A3（chromomycin A3，CMA）和 AT 特异性荧光染料 4'-6-二脒基-2-苯基吲哚（4'-6-diamidino-2-phenylindole，DAPI）染色。根据每个分类群的 22 条染色体的荧光带型，P. angularis、P. coccineus（来自中国和韩国）和 P. vulgaris 被分为一组（“菜豆属组”），P. aureus 和两个豇豆属物种分为另一组（“豇豆属组”），而 P. calcaratus 则分为一个独立的组。
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