Department of Plant Breeding, Punjab Agricultural University, 141004, Ludhiana, India.
Theor Appl Genet. 1993 Oct;87(1-2):106-12. doi: 10.1007/BF00223753.
Four near-isonuclear polycytoplasmic versions of 81A and two of Pb 402A male-sterile lines of pearl millet (Pennisetum typhoides) were used in factorial matings with five inbred male testers in different combinations in three sets. The cytoplasmic differences were studied for several agronomic traits using mean values and general combining effects (gca) of male-sterile lines, and specific combining ability effects of hybrids. The fertility/ sterility behaviour of different male-sterile lines in crosses with common male parents was also studied. Significant differences among near-isonuclear polycytoplasmic lines were observed in mean values for a few traits such as plant height, leaf length and peduncle length, but the differences for combining ability were more pronounced. The A3 cytoplasm was a better general combiner than the A2 cytoplasm for grain yield and both A2 and A3 cytoplasms were better general combiners for leaf length and peduncle length. In addition, superiority of A3 cytoplasm for gca was observed for plant height and ear characters over the A2 cytoplasm in set II. A differential behaviour of cytoplasms, both in combination with a common pollinator and across pollinators, was observed for several traits. The results provide evidence for the distinctiveness of different cytoplasmic sources in pearl millet and for the influence of cytoplasmic factors on the phenotypic expression of nuclear genes. A diversification of male sterility sources in the breeding of pearl millet hybrids is suggested.
四个近等核多质体 81A 和两个 Pb 402A 雄性不育系的珍珠粟（Pennisetum typhoides）与五个不同的自交雄性测验种在三组不同组合的析因交配中使用。使用雄性不育系的平均值和一般配合力（gca）以及杂种的特殊配合力效应，研究了细胞质差异对几个农艺性状的影响。还研究了不同雄性不育系与普通雄性亲本杂交的育性/不育行为。在几个性状如株高、叶片长度和花梗长度的平均值上观察到近等核多质体系之间存在显著差异，但在配合力方面的差异更为明显。A3 细胞质在产量方面比 A2 细胞质更适合一般组合，而 A2 和 A3 细胞质在叶片长度和花梗长度方面更适合一般组合。此外，在第二组中，A3 细胞质在株高和穗特征方面的 gca 优于 A2 细胞质。细胞质在与普通授粉者结合和跨授粉者结合方面的行为不同，对几个性状产生了影响。结果为珍珠粟中不同细胞质来源的独特性以及细胞质因素对核基因表型表达的影响提供了证据。建议在珍珠粟杂种的选育中多样化雄性不育源。
