College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
J Endocrinol. 2013 Dec 2;220(1):73-83. doi: 10.1530/JOE-13-0429. Print 2014 Jan.
Tryptophan 2,3-dioxygenase (Tdo2) is a rate-limiting enzyme which directs the conversion of tryptophan to kynurenine. The aim of this study was to examine the expression and regulation of Tdo2 in mouse uterus during decidualization. Tdo2 mRNA was mainly expressed in the decidua on days 6-8 of pregnancy. By real-time PCR, a high level of Tdo2 expression was observed in the uteri from days 6 to 8 of pregnancy, although Tdo2 expression was observed on days 1-8. Simultaneously, Tdo2 mRNA was also detected under in vivo and in vitro artificial decidualization. Estrogen, progesterone, and 8-bromoadenosine-cAMP could induce the expression of Tdo2 in the ovariectomized mouse uterus and uterine stromal cells. Tdo2 could regulate cell proliferation and stimulate the expression of decidual marker Dtprp in the uterine stromal cells and decidual cells. Overexpression of Tdo2 could upregulate the expression of Ahr, Cox2, and Vegf genes in uterine stromal cells, while Tdo2 inhibitor 680C91 could downregulate the expression of Cox2 and Vegf genes in uterine decidual cells. These data indicate that Tdo2 may play an important role during mouse decidualization and be regulated by estrogen, progesterone, and cAMP.
色氨酸 2,3-双加氧酶（Tdo2）是一种限速酶，它指导色氨酸转化为犬尿氨酸。本研究旨在探讨 Tdo2 在小鼠子宫蜕膜化过程中的表达和调节。Tdo2mRNA 主要在妊娠第 6-8 天的蜕膜中表达。通过实时 PCR，我们观察到妊娠第 6-8 天的子宫中 Tdo2 表达水平较高，尽管在第 1-8 天也观察到 Tdo2 表达。同时，在体内和体外人工蜕膜化下也检测到了 Tdo2mRNA。雌激素、孕激素和 8-溴腺苷-cAMP 可以诱导去卵巢小鼠子宫和子宫基质细胞中 Tdo2 的表达。Tdo2 可以调节细胞增殖，并刺激子宫基质细胞和蜕膜细胞中蜕膜标记物 Dtprp 的表达。Tdo2 的过表达可以上调子宫基质细胞中 Ahr、Cox2 和 Vegf 基因的表达，而 Tdo2 抑制剂 680C91 可以下调子宫蜕膜细胞中 Cox2 和 Vegf 基因的表达。这些数据表明，Tdo2 可能在小鼠蜕膜化过程中发挥重要作用，并受雌激素、孕激素和 cAMP 调节。
