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赤霉素诱导拟南芥中铁吸收相关基因的表达。

Gibberellin-induced expression of Fe uptake-related genes in Arabidopsis.

作者信息

Matsuoka Keita, Furukawa Jun, Bidadi Haniyeh, Asahina Masashi, Yamaguchi Shinjiro, Satoh Shinobu

机构信息

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572 Japan.

出版信息

Plant Cell Physiol. 2014 Jan;55(1):87-98. doi: 10.1093/pcp/pct160. Epub 2013 Nov 4.

Abstract

In dicots, iron (Fe) is acquired from the soil by IRT1 (IRON-REGULATED TRANSPORTER 1) and FRO2 (FERRIC REDUCTION OXIDASE 2) that are localized at the root epidermis. IRT1 and FRO2 expression is induced by local and systemic signals under Fe-deficient conditions in Arabidopsis thaliana. In this study, the expression of IRT1, FRO2, bHLH038 and bHLH39 (the latter two of which control IRT1 and FRO2 expression) was promoted by GA4 treatment of gibberellin (GA) deficient ga3ox1 ga3ox2 mutants. In contrast, the expression of FIT, which encodes a transcription factor necessary for IRT1 and FRO2 induction under Fe deficiency, was not induced by the application of GA4. The induction of those genes triggered by shoot-applied GA4 was observed, even in the fit-2 mutant which had reduced endogenous GA levels caused by treatment with paclobutrazol (PBZ), a GA biosynthesis inhibitor. These results suggested that FIT was not a key regulator in the GA responses under Fe-sufficient conditions. On the other hand, among Fe uptake-related genes, the expression of IRT1, bHLH038 and bHLH39 was lower in ga3ox1 ga3ox2 compared with the wild type (WT) under Fe-sufficient conditions, but the expression of all Fe uptake-related genes decreased under Fe-deficient conditions. Additionally, the PBZ treatment decreased IRT1 expression in the WT under Fe-deficient conditions, but not in the fit-2 mutant. These data suggest the contribution of GA to the induction of Fe uptake-related genes under Fe-sufficient and Fe-deficient conditions, possibly in FIT-independent and FIT-dependent manners, respectively.

摘要

在双子叶植物中,铁(Fe)通过定位在根表皮的IRT1(铁调节转运蛋白1)和FRO2(铁还原氧化酶2)从土壤中获取。在拟南芥中,缺铁条件下IRT1和FRO2的表达受局部和系统信号诱导。在本研究中,对赤霉素(GA)缺陷型ga3ox1 ga3ox2突变体进行GA4处理可促进IRT1、FRO2、bHLH038和bHLH39(后两者控制IRT1和FRO2的表达)的表达。相比之下,编码缺铁条件下诱导IRT1和FRO2所需转录因子的FIT的表达不受GA4处理的诱导。即使在用GA生物合成抑制剂多效唑(PBZ)处理导致内源GA水平降低的fit-2突变体中,也观察到了由地上部施用GA4引发的那些基因的诱导。这些结果表明,在铁充足的条件下,FIT不是GA反应中的关键调节因子。另一方面,在铁吸收相关基因中,在铁充足的条件下,ga3ox1 ga3ox2中IRT1、bHLH038和bHLH39的表达低于野生型(WT),但在缺铁条件下所有铁吸收相关基因的表达均下降。此外,PBZ处理在缺铁条件下降低了WT中IRT1的表达,但在fit-2突变体中未降低。这些数据表明,GA可能分别以不依赖FIT和依赖FIT的方式,在铁充足和缺铁条件下对铁吸收相关基因的诱导起作用。

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