Du Ying-Chun, Kong Ling-Jie, Cao Ling-Sen, Zhang Wei, Zhu Qiang, Ma Chen-Yu, Sun Kai, Dai Chuan-Chao
Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology and Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Front Plant Sci. 2022 Apr 29;13:872242. doi: 10.3389/fpls.2022.872242. eCollection 2022.
Iron (Fe) deficiency in alkaline calcium soil is a problem that needs to be solved urgently as Fe is an essential and commonly limiting nutrient for plants. Endophytic fungus, (), has been reported to promote Fe absorption in peanuts ( L.), however, the mechanisms remain unclear. Under prolonged Fe deficiency, an increase in hydrogen peroxide (HO) often triggers a series of signaling events and leads to the inhibition of Fe acquisition. The main purpose of this study was to explore whether and how the endophytic fungus promote Fe absorption in peanut through regulating HO and assisting in resisting oxidative stress. In this study, we detected the Fe deficiency-induced transcription factor (), Fe transporter (1), and ferric reduction oxidase 2 () of peanuts, and confirmed that they were negatively related to Fe concentration. Similarly, 1, and were also inhibited by HO. The addition of reduces HO under Fe-deficiency with an increase in Fe content, while the exogenous addition of HO further decreases it, and the addition of catalase (CAT) under Fe-deficiency reverses this phenomenon. Through transcriptome analysis, we proved that the expression of 1, and CAT are consistent with our hypothesis, and has a stress-mitigating effect on peanuts mainly via CAT, glutathione peroxidase, and malondialdehyde. Our study proved the Fe-absorption promoting effect and stress mitigation effect of under Fe-deficiency in peanuts, and their combined usage may help peanuts grow better.
在碱性钙质土壤中，铁（Fe）缺乏是一个亟待解决的问题，因为铁是植物必需且普遍受限的养分。据报道，内生真菌（）可促进花生（）对铁的吸收，但其机制尚不清楚。在长期缺铁的情况下，过氧化氢（HO）的增加通常会引发一系列信号事件，并导致铁吸收受到抑制。本研究的主要目的是探讨内生真菌是否以及如何通过调节HO和协助抵抗氧化应激来促进花生对铁的吸收。在本研究中，我们检测了花生缺铁诱导的转录因子（）、铁转运蛋白（1）和铁还原氧化酶2（），并证实它们与铁浓度呈负相关。同样，1和也受到HO的抑制。在缺铁条件下添加可降低HO并增加铁含量，而外源添加HO则进一步降低铁含量，在缺铁条件下添加过氧化氢酶（CAT）可逆转这一现象。通过转录组分析，我们证明1、和CAT的表达与我们的假设一致，并且主要通过CAT、谷胱甘肽过氧化物酶和丙二醛对花生具有减轻胁迫的作用。我们的研究证明了在花生缺铁条件下具有促进铁吸收和减轻胁迫的作用，它们的联合使用可能有助于花生更好地生长。
