Del Buono B J, Williamson P L, Schlegel R A
J Cell Physiol. 1986 Mar;126(3):379-88. doi: 10.1002/jcp.1041260308.
The fluorescent probe merocyanine 540, which binds preferentially to bilayers in which the lipids are loosely packed, was used to investigate changes in the organization of the lipids of the lymphocyte plasma membrane during primary and secondary lymphopoiesis. When mouse thymocytes were incubated with the dye, most immature cells stained, while most mature cells, about to enter the peripheral circulation, did not. Similarly, mature lymphocytes from both mouse and human peripheral blood did not stain, but these same cells did when activated by in vitro mitogenic stimulation. Freshly isolated splenic lymphocytes, presumably activated in vivo by antigen, also bound merocyanine 540, but after 48 hours of culture in the absence of stimulus they displayed only a low affinity for the dye, a phenotype that reverted to a high affinity upon mitogenic stimulation. These results suggest that changes in the organization of the lipids of the plasma membrane take place during lymphocyte differentiation: viz., immature cells possess a disordered membrane that becomes increasingly ordered as the cells mature and enter the peripheral circulation; then, upon antigen-induced differentiation, the plasma membrane again becomes disordered. These lipid organization changes are discussed in the context of their possible role in the regulation of lymphocyte circulation via intercellular interactions between lymphocytes and cells of the reticuloendothelial system.
荧光探针部花青540优先结合脂质排列疏松的双层膜，被用于研究在初级和次级淋巴细胞生成过程中淋巴细胞质膜脂质组织的变化。当用该染料孵育小鼠胸腺细胞时，大多数未成熟细胞被染色，而大多数即将进入外周循环的成熟细胞未被染色。同样，来自小鼠和人外周血的成熟淋巴细胞未被染色，但这些相同的细胞在体外有丝分裂原刺激下被激活时会被染色。新鲜分离的脾淋巴细胞，可能在体内被抗原激活，也结合部花青540，但在无刺激条件下培养48小时后，它们对该染料仅表现出低亲和力，这种表型在有丝分裂原刺激后又恢复为高亲和力。这些结果表明，在淋巴细胞分化过程中质膜脂质组织发生了变化：即未成熟细胞具有无序的膜，随着细胞成熟并进入外周循环，膜变得越来越有序；然后，在抗原诱导的分化过程中，质膜再次变得无序。本文结合这些脂质组织变化在淋巴细胞与网状内皮系统细胞间相互作用调节淋巴细胞循环中可能发挥的作用进行了讨论。
