Redburn D A, Madtes P
J Comp Neurol. 1986 Jan 1;243(1):41-57. doi: 10.1002/cne.902430105.
Light and electron microscopic autoradiography demonstrates that 3H-GABA is accumulated by horizontal cells in neonatal rabbit retina but not in the adult. A specific population of horizontal cells appears to be mature at birth and they avidly accumulate 3H-GABA during a 15-minute incubation period in vitro. Uptake into horizontal cells is not observed after the fifth postnatal day; 3H-GABA-accumulating horizontal cell bodies and their processes are the first identifiable components that clearly mark the future location of the outer plexiform layer at birth and as such, may be considered pioneering elements. Our observations raise the interesting possibility that the pioneering horizontal cell may provide structural and/or chemical factors necessary for the subsequent development of the outer plexiform layer of the retina. Labeling patterns of other retinal cells also show varying degrees of change during development. A population of amacrine cells accumulate 3H-GABA at birth. These cells show little change in their morphological or 3H-GABA uptake properties from birth to adulthood. Müller cells show weak accumulation of 3H-GABA at birth. Subsequent to this time, labeling of Müller cells is significantly more robust, resulting in Müller cell domination of retinal autoradiographic patterns in more mature retinas. Every cell body in the ganglion cell layer accumulates 3H-GABA at birth. The number of labeled cells declines during postnatal development, resulting in a very limited adult population. We conclude that the ability of retinal cells to accumulate 3H-GABA does not remain constant during postnatal development; rather each cell population displays a unique maturation sequence that results in a dramatic developmental shift in the number and types of GABA-accumulating cells present in the retina.
光学显微镜和电子显微镜放射自显影表明，新生兔视网膜中的水平细胞可积累³H - GABA，而成年兔视网膜中的水平细胞则不能。特定群体的水平细胞在出生时似乎就已成熟，在体外15分钟的孵育期内它们能大量积累³H - GABA。出生后第5天之后就观察不到³H - GABA进入水平细胞的情况；积累³H - GABA的水平细胞体及其突起是出生时最早可识别的成分，它们清晰地标记了未来外网状层的位置，因此可被视为先驱元素。我们的观察结果提出了一个有趣的可能性，即先驱水平细胞可能为视网膜外网状层的后续发育提供必要的结构和/或化学因素。视网膜其他细胞的标记模式在发育过程中也显示出不同程度的变化。一群无长突细胞在出生时积累³H - GABA。从出生到成年，这些细胞的形态或³H - GABA摄取特性几乎没有变化。米勒细胞在出生时³H - GABA积累较弱。在此之后，米勒细胞的标记明显更强，导致在更成熟的视网膜中米勒细胞主导了视网膜放射自显影模式。神经节细胞层中的每个细胞体在出生时都积累³H - GABA。出生后发育过程中标记细胞的数量减少，导致成年群体非常有限。我们得出结论，视网膜细胞积累³H - GABA的能力在出生后发育过程中并非保持不变；相反，每个细胞群体都表现出独特的成熟序列，这导致视网膜中积累GABA的细胞数量和类型发生显著的发育变化。
