Institute of Plant Breeding, University of the Philippines at Los Baños, College, 4031, Laguna, Philippines.
Theor Appl Genet. 1993 Aug;86(7):797-810. doi: 10.1007/BF00212605.
Genome relationships between mungbean (Vigna tradiata) and cowpea (V. Unguiculata) based on the linkage arrangement of random genomic restriction fragment length polymorphism (RFLP) markers have been investigated. A common set of probes derived from cowpea, common bean (Phaseolus vulgaris), mungbean, and soybean (Glycine max) PstI genomic libraries were used to construct the genetic linkage maps. In both species, a single F2 population from a cross between an improved cultivar and a putative wild progenitor species was used to follow the segregation of the RFLP markers. Approximately 90% of the probes hybridized to both mungbean and cowpea DNA, indicating a high degree of similarity in the nucleotide sequences among these species. A higher level of polymorphism was detected in the mungbean population (75.7%) than in the cowpea population (41.2%). Loci exhibiting duplications, null phenotypes, and distorted segregation ratios were detected in both populations. Random genomic DNA RFLP loci account for about 89% of the currently mapped markers with a few cDNA and RAPD markers added. The current mungbean map is comprised of 171 loci/loci clusters distributed in 14 linkage groups spanning a total of 1570cM. On the other hand, 97 markers covered 684 cM and defined 10 linkage groups in the current cowpea map. The mungbean and cowpea genomes were compared on the basis of the copy number and linkage arrangement of 53 markers mapped in common between the two species. Results indicate that nucleotide sequences are conserved, but variation in copy number were detected and several rearrangements in linkage orders appeared to have occurred since the divergence of the two species. Entire linkage groups were not conserved, but several large linkage blocks were maintained in both genomes.
基于随机基因组限制性片段长度多态性（RFLP）标记的连锁排列，研究了绿豆（Vigna radiata）和豇豆（V. unguiculata）之间的基因组关系。从豇豆、普通菜豆（Phaseolus vulgaris）、绿豆和大豆（Glycine max）PstI 基因组文库中衍生出的一组共同探针被用于构建遗传连锁图谱。在这两个物种中，均使用来自改良品种和假定野生祖先种之间杂交的单个 F2 群体来跟踪 RFLP 标记的分离。大约 90%的探针与绿豆和豇豆 DNA 杂交，表明这些物种的核苷酸序列高度相似。在绿豆群体中检测到的多态性水平（75.7%）高于豇豆群体（41.2%）。在两个群体中都检测到了具有重复、空表型和偏分离比的位点。随机基因组 DNA RFLP 位点约占当前映射标记的 89%，并添加了一些 cDNA 和 RAPD 标记。当前的绿豆图谱由 171 个位点/位点簇组成，分布在 14 个连锁群中，总跨度为 1570cM。另一方面，97 个标记覆盖了 684cM，并在当前的豇豆图谱中定义了 10 个连锁群。在两个物种之间映射的 53 个标记的数量和连锁排列的基础上，对绿豆和豇豆基因组进行了比较。结果表明，核苷酸序列是保守的，但在拷贝数上存在差异，并且在两个物种分化后，出现了一些连锁顺序的重排。整个连锁群没有被保守，但在两个基因组中都保持了几个大的连锁块。
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