Departmento de Biología Aplicada, Escuela Politécnica Superior, Universidad de Almería, 04120, Almería, Spain.
Fish Physiol Biochem. 1996 Apr;15(2):121-30. doi: 10.1007/BF01875591.
The evolution of the digestive enzyme equipment in seabream from hatching to 30 days old larvae was studied; there was a progressive increase in the activity of protease, amylase and acid and alkaline phosphatase from day 15 onwards. The use of specific inhibitors, and SDS-PAGE provided evidence to suggest that most of the proteases belonged to the serine group. A high α-amylase activity was also denoted. Zymograms of larval extracts indicated that exogenous food has more a qualitative than a quantitative role in the secretion of digestive enzymes in this species.
研究了从孵化到 30 天大的鲈鱼幼鱼的消化酶设备的演变；从第 15 天开始，蛋白酶、淀粉酶和酸碱性磷酸酶的活性逐渐增加。使用特定的抑制剂和 SDS-PAGE 提供了证据表明，大多数蛋白酶属于丝氨酸组。还指出了高α-淀粉酶活性。幼虫提取物的同工酶图谱表明，外源性食物在该物种消化酶的分泌中具有更多的定性作用而不是定量作用。
