胸腺巨噬细胞移动抑制因子（MIF）的纯化。
Purification of thymic macrophage migration inhibitory factor (MIF).
机构信息
Biochemical Research Laboratory, Research Foundation of Children's Hospital, National Medical Center, Washington, D.C..
出版信息
Inflammation. 1976 Mar;1(2):189-200. doi: 10.1007/BF00917529.
Aqueous extracts of the thymus of animals which had been challenged immunologically have been shown to contain MIF activity. This MIF could be purified by precipitation with 70% ethanol, concentrated by ultrafiltration between 30,000 and 50,000 daltons, isoelectrically focused at pH 6.8-7.1, and electrophoresed on preparative acrylamide gels. The resulting product is electrophoretically homogeneous at pH 4.3 in polyacrylamide-gel electrophoresis and SDS-gel electrophoresis. It has a molecular weight of 36,000 daltons. It is trypsin- and neuraminidase-labile and is thermostable. It degrades and reassembles in electrophoresis at pH 7.0. It is not chemotactic for macrophages but apparently activates them phagocytically. It has no proteolytic activity.
经免疫挑战的动物胸腺的水提取物已被证明含有 MIF 活性。这种 MIF 可以通过用 70%乙醇沉淀来纯化，用 30,000 到 50,000 道尔顿之间的超滤浓缩，在 pH 6.8-7.1 等电聚焦，然后在制备性丙烯酰胺凝胶上电泳。在 pH 4.3 的聚丙烯酰胺凝胶电泳和 SDS 凝胶电泳中，得到的产物电泳均一。它的分子量为 36000 道尔顿。它对胰蛋白酶和神经氨酸酶敏感，并且热稳定。它在 pH 7.0 的电泳中降解和重新组装。它对巨噬细胞没有趋化作用，但显然可以通过吞噬作用激活它们。它没有蛋白水解活性。
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