链脲佐菌素诱导的糖尿病会降低大鼠大脑中血清素的合成。

Streptozotocin-induced diabetes reduces brain serotonin synthesis in rats.

作者信息

Trulson M E, Jacoby J H, MacKenzie R G

出版信息

J Neurochem. 1986 Apr;46(4):1068-72. doi: 10.1111/j.1471-4159.1986.tb00619.x.

DOI:10.1111/j.1471-4159.1986.tb00619.x

PMID:2419504

Abstract

The rate of brain 5-hydroxytryptamine (serotonin) synthesis and turnover in streptozotocin-diabetic rats was assessed using three separate methods: the rate of 5-hydroxytryptophan accumulation following decarboxylase inhibition with Ro 4-4602; the decline in 5-hydroxyindoleacetic acid levels following monoamine oxidase inhibition with pargyline; and the rate of 5-hydroxyindoleacetic acid accumulation following blockade of acid transport with probenecid. Each of the three methods revealed that 5-hydroxytryptamine synthesis and turnover is decreased by 44-71% in diabetic rats with plasma glucose levels of between 500 and 600 mg%. In addition, the levels of free and bound plasma tryptophan were measured and the levels of the free amino acid were found to be the same in control and diabetic rats. Since diabetic rats exhibit a 40% decrease in brain tryptophan, the free tryptophan level in plasma does not predict brain tryptophan levels in diabetic rats. These data are discussed within the context of psychiatric disturbances experienced by diabetic patients.

摘要

采用三种不同方法评估链脲佐菌素诱导的糖尿病大鼠脑内5-羟色胺（血清素）的合成及更新率：用Ro 4-4602抑制脱羧酶后5-羟色氨酸的积累率；用优降宁抑制单胺氧化酶后5-羟吲哚乙酸水平的下降；用丙磺舒阻断酸转运后5-羟吲哚乙酸的积累率。这三种方法均显示，血浆葡萄糖水平在500至600mg%之间的糖尿病大鼠，其5-羟色胺的合成及更新率降低了44%-71%。此外，还测量了游离和结合的血浆色氨酸水平，发现对照组和糖尿病大鼠的游离氨基酸水平相同。由于糖尿病大鼠脑内色氨酸减少了40%，因此血浆中游离色氨酸水平并不能预测糖尿病大鼠脑内色氨酸水平。本文结合糖尿病患者经历的精神障碍对这些数据进行了讨论。