油菜（甘蓝型油菜亚种甘蓝型油菜）小孢子培养及新鲜和条件培养基的影响。

Microspore culture of swede (Brassica napus ssp. rapifera) and the effects of fresh and conditioned media.

机构信息

Department of Horticulture, Agricultural Univ. of Norway, P.O. Box 5022, N-1432 Ås, Norway.

出版信息

Plant Cell Rep. 1993 Jul;12(9):496-500. doi: 10.1007/BF00236094.

PMID:24196108

Abstract

Microspore culture was successfully carried out with four cultivars of swede (Brassica napus ssp. rapifera). Embryo yields increased markedly if the culture medium was replaced by fresh medium after a culture period of three days, with more than 200% increase with the highly responding cultivar Gry and more than 800% with the poorly responding cultivar Stenhaug. If the medium was changed at a later time, embryo production declined sharply. Transfer of microspores after 3 days of culture to a medium in which microspores of a different cultivar had been cultured for 3 days had no effect on embryogenesis. Bud size had a large effect on embryo production, particularly with the least responsive cultivar, Stenhaug.

摘要

小孢子培养在四个芜菁（甘蓝型油菜亚种黑芥）品种中成功进行。如果在培养三天后用新鲜培养基代替培养基，胚胎产量会显著增加，其中高响应品种 Gry 增加超过 200%，低响应品种 stenhaug 增加超过 800%。如果在稍后的时间更换培养基，胚胎产量会急剧下降。将培养 3 天后的小孢子转移到另一个已培养 3 天的不同品种小孢子的培养基中，对胚胎发生没有影响。芽大小对胚胎产量有很大影响，特别是在响应性最低的品种 stenhaug 中。