Weil M L, Menkes J H
Pediatr Res. 1975 Oct;9(10):791-3. doi: 10.1203/00006450-197510000-00009.
Reaction of bilirubin with increasing amounts of ganglioside purified from neonatal brain significantly alters the spectral absorption of bilirubin in proportion to the quantity of ganglioside added. Increments in absorbance occur at 353 nm with a prompt but transient increase at 486 nm. A decrease in absorbance occurs which is most marked at 447 nm. When gangliosides are added to bilirubin (9.1 mug/ml or 0.016 muM/ml), the decrease in absorbance is essentially linear up to the highest concentration of purified ganglioside tested (182 mug/ml or 0.097 muM/ml), which represents a molar ratio of 6.1:1. The asymptotic nature of the bilirubin-ganglioside reaction as measured by the decrease in absorbance with time suggests a stoichiometric relationship between the two substances. An isosbestic point was demonstrated at 405 nm. Observations reported here suggest bilirubin reaction with ganglioside is at least a two-step process.
胆红素与从新生动物大脑中纯化出的越来越多的神经节苷脂反应，会使胆红素的光谱吸收按照所添加神经节苷脂的量成比例地发生显著改变。在353nm处吸光度增加，在486nm处有迅速但短暂的增加。在447nm处吸光度下降最为明显。当向胆红素（9.1微克/毫升或0.016微摩尔/毫升）中添加神经节苷脂时，直至所测试的纯化神经节苷脂的最高浓度（182微克/毫升或0.097微摩尔/毫升，摩尔比为6.1:1），吸光度的下降基本呈线性。通过吸光度随时间的下降来衡量，胆红素 - 神经节苷脂反应的渐近性质表明这两种物质之间存在化学计量关系。在405nm处显示出一个等吸收点。此处报告的观察结果表明胆红素与神经节苷脂的反应至少是一个两步过程。
